BMRB Entry 7073
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7073
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NMR-STAR v3 text file.
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Citation: Mayer, Kristen; Qu, Ying; Bansal, Sonal; LeBlond, Peter; Jenney, Frank; Brereton, P.; Adams, Mike; Xu, Ying; Prestegard, James. "Structure determination of a new protein from backbone-centered NMR data and
NMR-assisted structure prediction" Proteins 65, 480-489 (2006).
PubMed: 16927360
Assembly members:
PF1455, polymer, 82 residues, Formula weight is not available
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PF1455: AHHHHHHGSKWIKFTTNLTP
EEAKIVQYELSTRDEFYRVF
INPYAKVAEVVIDDSKVNIE
ELKEKLKGEVIEEKEITLEE
LI
- assigned_chemical_shifts
- coupling_constants
- RDCs
| Data type | Count |
| 13C chemical shifts | 69 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 112 |
| coupling constants | 59 |
| residual dipolar couplings | 172 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pfam | 1 |
Entities:
Entity 1, Pfam 82 residues - Formula weight is not available
| 1 | ALA | HIS | HIS | HIS | HIS | HIS | HIS | GLY | SER | LYS | ||||
| 2 | TRP | ILE | LYS | PHE | THR | THR | ASN | LEU | THR | PRO | ||||
| 3 | GLU | GLU | ALA | LYS | ILE | VAL | GLN | TYR | GLU | LEU | ||||
| 4 | SER | THR | ARG | ASP | GLU | PHE | TYR | ARG | VAL | PHE | ||||
| 5 | ILE | ASN | PRO | TYR | ALA | LYS | VAL | ALA | GLU | VAL | ||||
| 6 | VAL | ILE | ASP | ASP | SER | LYS | VAL | ASN | ILE | GLU | ||||
| 7 | GLU | LEU | LYS | GLU | LYS | LEU | LYS | GLY | GLU | VAL | ||||
| 8 | ILE | GLU | GLU | LYS | GLU | ILE | THR | LEU | GLU | GLU | ||||
| 9 | LEU | ILE |
Samples:
sample_3: PF1455, [U-15N; U-16% 13C], 1.2 mM; Tris 20 mM; NaCl 70 mM; C12E5/hexanol 3%
sample_1: PF1455, [U-15N], 2 mM; Tris 20 mM; NaCl 70 mM
sample_2: PF1455, [U-15N; U-16% 13C], 1.14 mM; Tris 11 mM; NaCl 40 mM; Phage 10 mg/ml
conditions_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N IPAP HSQC_(example) | not available | not available | not available |
| HNHA | not available | not available | conditions_1 |
| soft HNCA ECOSY | not available | not available | conditions_1 |
| NOESY-15N edited | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks