BMRB Entry 7072

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7072

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the PDZ domain of GOPC
Deposition date:
2006-04-14
Original release date:
2006-04-24
Authors:
Li, Xiang; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu
Citation:

Citation: Li, Xiang; Zhang, Jiahai; Cao, Zanxia; Wu, Jihui; Shi, Yunyu. "Solution Structure of GOPC PDZ Domain and Its Interaction with the C-Terminal motif of Neuroligin"  Protein Sci. 15, 2149-2158 (2006).
PubMed: 16882988

Assembly members:

Assembly members:
GOPC PDZ domain, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: homo spaiens   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo spaiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET22(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GOPC PDZ domain: PIRKVLLLKEDHEGLGISIT GGKEHGVPILISEIHPGQPA DRCGGLHVGDAILAVNGVNL RDTKHKEAVTILSQQRGEIE FEVVYVA

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts87
1H chemical shifts621

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GOPC PDZ monomer1

Entities:

Entity 1, GOPC PDZ monomer 87 residues - Formula weight is not available

1   PROILEARGLYSVALLEULEULEULYSGLU
2   ASPHISGLUGLYLEUGLYILESERILETHR
3   GLYGLYLYSGLUHISGLYVALPROILELEU
4   ILESERGLUILEHISPROGLYGLNPROALA
5   ASPARGCYSGLYGLYLEUHISVALGLYASP
6   ALAILELEUALAVALASNGLYVALASNLEU
7   ARGASPTHRLYSHISLYSGLUALAVALTHR
8   ILELEUSERGLNGLNARGGLYGLUILEGLU
9   PHEGLUVALVALTYRVALALA

Samples:

sample_1: GOPC PDZ domain, [U-15N], 1.0 ± 0.1 mM; phosphate buffer 20 ± 0.5 mM; EDTA 1 ± 0.1 mM; DTT 4 ± 0.1 mM; D2O 10 ± 0.5 %

sample_2: GOPC PDZ domain, [U-13C; U-15N], 1.0 ± 0.1 mM; phosphate buffer 20 ± 0.5 mM; EDTA 1 ± 0.1 mM; DTT 4 ± 0.1 mM; D2O 10 ± 0.5 %

conditions_1: pH: 6.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
CBCANHnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HBHACBCACONHnot availablenot availableconditions_1
HCCONH-tocsynot availablenot availableconditions_1
CCONH-tocsynot availablenot availableconditions_1
15N_NOESYnot availablenot availableconditions_1
HNCACOnot availablenot availableconditions_1
13C_NOESYnot availablenot availableconditions_1
HCCH-tocsynot availablenot availableconditions_1
HCCH-cosynot availablenot availableconditions_1

Software:

SPARKY v3 -

NMRPipe v2.3 -

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 18205
PDB
DBJ BAB69946 BAC26958 BAC27058 BAC27951
EMBL CAH90325
GB AAG00571 AAG00572 AAH09553 AAH51171 AAI68160
PRF 1611455A
REF NP_001017408 NP_001101101 NP_001125151 NP_001186201 NP_001193086
SP Q5RD32 Q8BH60 Q9HD26
TPG DAA26316 DAA26317
AlphaFold Q5RD32 Q8BH60 Q9HD26

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks