BMRB Entry 7071

Name: Backbone and Beta 1H, 13C, and 15N Chemical Shift Assignments for the Bacterial Antitoxin Kis
Published: 2007-04-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7071

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and Beta 1H, 13C, and 15N Chemical Shift Assignments for the Bacterial Antitoxin Kis

Deposition date:

2006-04-13

Original release date:

2007-04-11

Authors:

Kamphuis, Monique; Boelens, Rolf

Citation:

Citation: Kamphuis, Monique; Monti, M.; van den Heuvel, R.; Santos-Sierra, S.; Folkers, G.; Lemonnier, M.; Diaz-Orejas, R.; Heck, A.; Boelens, Rolf. "Interactions between the toxin kid of the bacterial parD system and the antitoxins Kis and MazE" Proteins 67, 219-231 (2007).
PubMed: 17206710

Assembly members:

Assembly members:
Kis antitoxin, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Kis antitoxin: GSMHTTRLKRVGGSVMLTVP PALLNALSLGTDNEVGMVID NGRLIVEPYRRPQYSLAELL AQCDPNAEISAEEREWLDAP ATGQEEI

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	241
15N chemical shifts	80
1H chemical shifts	310

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Kis antitoxin	1

Entities:

Entity 1, Kis antitoxin 87 residues - Formula weight is not available

1

GLY

SER

MET

HIS

THR

ARG

LEU

LYS

ARG

2

VAL

GLY

SER

VAL

MET

LEU

THR

VAL

PRO

3

PRO

ALA

LEU

ASN

ALA

LEU

SER

LEU

GLY

4

THR

ASP

ASN

GLU

VAL

GLY

MET

VAL

ILE

ASP

5

ASN

GLY

ARG

LEU

ILE

VAL

GLU

PRO

TYR

ARG

6

ARG

PRO

GLN

TYR

SER

LEU

ALA

GLU

LEU

7

ALA

GLN

CYS

ASP

PRO

ASN

ALA

GLU

ILE

SER

8

ALA

GLU

ARG

GLU

TRP

LEU

ASP

ALA

PRO

9

ALA

THR

GLY

GLN

GLU

ILE

Samples:

sample_1: Kis antitoxin, [U-13C; U-15N], 0.4 mM; PO4 (K) 100 mM

conditions_1: pH: 5.8; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	not available	conditions_1
2D 1H-13C HSQC	sample_1	not available	conditions_1
3D HNCA	sample_1	not available	conditions_1
3D HN(CO)CA	sample_1	not available	conditions_1
3D HNCACB	sample_1	not available	conditions_1
3D CBA(CO)NH	sample_1	not available	conditions_1
3D HNCO	sample_1	not available	conditions_1
3D HA(CA)NH	sample_1	not available	conditions_1
3D HBA(CO)NH	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker AVANCE 600 MHz

DBJ	BAA78897 BAL45865 BAL48675 BAN28645 BAP10672
EMBL	CAA29584 CAH64730 CAP07762 CAQ87432 CBG91767
GB	AAA26069 AAO49557 AAQ97876 AAR05735 AAR25127
REF	NP_052993 NP_862962 NP_943201 NP_957646 WP_000557618
SP	P13975
AlphaFold	P13975