BMRB Entry 7067

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7067
MolProbity Validation Chart

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Title:
Human Nogo-A functional domain: nogo60
Deposition date:
2006-04-10
Original release date:
2006-11-17
Authors:
Li, M.; Liu, J.; Song, J.
Citation:

Citation: Li, M.; Liu, J.; Song, J.. "Nogo goes in the pure water: solution structure of Nogo-60 and design of the structured and buffer-soluble Nogo-54 for enhancing CNS regeneration"  Protein Sci. 15, 1835-1841 (2006).
PubMed: 16877707

Assembly members:

Assembly members:
Reticulon-4, polymer, 60 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Reticulon-4: RIYKGVIQAIQKSDEGHPFR AYLESEVAISEELVQKYSNS ALGHVNCTIKELRRLFLVDD

Data sets:
Data typeCount
15N chemical shifts57
1H chemical shifts302

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Reticulon-41

Entities:

Entity 1, Reticulon-4 60 residues - Formula weight is not available

1   ARGILETYRLYSGLYVALILEGLNALAILE
2   GLNLYSSERASPGLUGLYHISPROPHEARG
3   ALATYRLEUGLUSERGLUVALALAILESER
4   GLUGLULEUVALGLNLYSTYRSERASNSER
5   ALALEUGLYHISVALASNCYSTHRILELYS
6   GLULEUARGARGLEUPHELEUVALASPASP

Samples:

sample_1: Reticulon-4; H2O 90%; D2O 10%

sample_cond_1: pH: 4.0; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available

Software:

DYANA vCYANA 1.0.6 - refinement

NMR spectrometers:

  • Bruker INOVA 800 MHz

Related Database Links:

BMRB 15933 16257
PDB
DBJ BAA74909 BAA83712 BAB16739 BAB18927 BAB18928
EMBL CAB71027 CAB71028 CAB71029 CAB99248 CAB99249
GB AAD27783 AAD31019 AAD31020 AAD31021 AAD31022
REF NP_001029083 NP_001068606 NP_001106692 NP_001123435 NP_001126875
SP Q99P72 Q9JK11 Q9NQC3
TPG DAA01940 DAA01967 DAA01973 DAA02035 DAA05710
AlphaFold Q99P72 Q9JK11 Q9NQC3

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks