BMRB Entry 7061

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PDB ID: 2g35
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7061
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of talin-PTB in complex with PIPKI

Deposition date:

2006-04-10

Original release date:

2008-07-17

Authors:

Kong, X.; Wang, X.; Misra, S.; Qin, J.

Citation:

Citation: Kong, X.; Wang, X.; Misra, S.; Qin, J.. "Structural Basis for the Phosphorylation-regulated Focal Adhesion Targeting of Type Igamma Phosphatidylinositol Phosphate Kinase (PIPKIgamma) by Talin" J. Mol. Biol. 359, 47-54 (2006).
PubMed: 16616931

Assembly members:

Assembly members:
Talin-1, polymer, 106 residues, Formula weight is not available
SWVXSPLH, polymer, 8 residues, 261.168 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Talin-1: LKTYGVSFFLVKEKMKGKNK LVPRLLGITKECVMRVDEKT KEVIQEWSLTNIKRWAASPK SFTLDFGDYQDGYYSVQTTE GEQIAQLIAGYIDIILKKKK SKDHFG
SWVXSPLH: SWVXSPLH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
- chemical_shift_set_2

Data type	Count
13C chemical shifts	441
15N chemical shifts	123
1H chemical shifts	740

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Talin-1	1
2	peptide	2

Entities:

Entity 1, Talin-1 106 residues - Formula weight is not available

1

LEU

LYS

THR

TYR

GLY

VAL

SER

PHE

LEU

2

VAL

LYS

GLU

LYS

MET

LYS

GLY

LYS

ASN

LYS

3

LEU

VAL

PRO

ARG

LEU

GLY

ILE

THR

LYS

4

GLU

CYS

VAL

MET

ARG

VAL

ASP

GLU

LYS

THR

5

LYS

GLU

VAL

ILE

GLN

GLU

TRP

SER

LEU

THR

6

ASN

ILE

LYS

ARG

TRP

ALA

SER

PRO

LYS

7

SER

PHE

THR

LEU

ASP

PHE

GLY

ASP

TYR

GLN

8

ASP

GLY

TYR

SER

VAL

GLN

THR

GLU

9

GLY

GLU

GLN

ILE

ALA

GLN

LEU

ILE

ALA

GLY

10

TYR

ILE

ASP

ILE

LEU

LYS

11

SER

LYS

ASP

HIS

PHE

GLY

Entity 2, peptide 8 residues - 261.168 Da.

1

SER

TRP

VAL

PTR

SER

PRO

LEU

HIS

Samples:

sample_1: Talin-1, [U-13C; U-15N], 1 mM; SWVXSPLH 1 mM; phosphate buffer 20 mM; Ca2+ 5 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 6.3; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCACB	sample_1	not available	sample_cond_1
CBCA(CO)NH	sample_1	not available	sample_cond_1
HCCH-TOCSY	sample_1	not available	sample_cond_1
HNCO	sample_1	not available	sample_cond_1
edited NOESY	sample_1	not available	sample_cond_1
filtered NOESY	sample_1	not available	sample_cond_1

Software:

TALOS - data analysis

X-PLOR - refinement

NMR spectrometers:

VARIAN INOVA 600 MHz

BMRB	15792
PDB	1MIX 1MIZ 1MK7 1MK9 1Y19 2G35 2H7D 2H7E 2K00 2KGX 3IVF
DBJ	BAA82979 BAC30516 BAC65702 BAE27781 BAG09941
EMBL	CAA39588 CAI10978 CAM17190
GB	AAD13152 AAF23322 AAF27330 AAH42923 AAI21760
PRF	1617167A
REF	NP_001034114 NP_006280 NP_035732 NP_989854 XP_001084941
SP	P26039 P54939 Q9Y490
AlphaFold	P26039 P54939 Q9Y490