BMRB Entry 7057

Title:
Chemical Shift Assignment for hbSBD
Deposition date:
2006-04-08
Original release date:
2008-10-27
Authors:
Lin, Yi-Jan; Chang, Chi-Fon; Chuang, David T.; Huang, Tai-huang
Citation:

Citation: Chang, Chi-Fon; Chou, Hui-Ting; Lin, Yi-Jan; Lee, Shin-Jye; Chuang, Jacinta L.; Chuang, David T.; Huang, Tai-huang. "Structure of the subunit binding domain and dynamics of the di-domain region from the core of human branched chain alpha-ketoacid dehydrogenase complex."  J. Biol. Chem. 281, 28345-28353 (2006).
PubMed: 16861235

Assembly members:

Assembly members:
hbSBD, polymer, 52 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts154
15N chemical shifts46
1H chemical shifts309

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hbSBD monomer1

Entities:

Entity 1, hbSBD monomer 52 residues - Formula weight is not available

1   GLYGLUILELYSGLYARGLYSTHRLEUALA
2   THRPROALAVALARGARGLEUALAMETGLU
3   ASNASNILELYSLEUSERGLUVALVALGLY
4   SERGLYLYSASPGLYARGILELEULYSGLU
5   ASPILELEUASNTYRLEUGLULYSGLNTHR
6   LEUGLU

Samples:

sample_1: hbSBD, [U-13C; U-15N], 1 mM; phosphate buffer1 – 2 mM; NaCl 100 mM

conditions_1: ionic strength: 100 mM; pH: 7.5; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
TOCSY-HSQCsample_1not availableconditions_1
NOESY-HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11240
PDB
DBJ BAF62349 BAG36008 BAG64869
EMBL CAA47285 CAH91978
GB AAA35589 AAA59200 AAA64512 AAH16675 AAP36036
REF NP_001127490 NP_001136231 NP_001248206 NP_001909 XP_001488662
SP P11182
AlphaFold P11182

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks