BMRB Entry 7053
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7053
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Jaremko, Lukasz; Jaremko, Mariusz; Filipek, Renata; Wojciechowski, Marek; Szczepanowski, Roman; Bochtler, Matthias; Zhukov, Igor. "NMR assignment of a structurally uncharacterised fragment of recombinant mouse
ubiquitin-activating enzyme" J. Biomol. NMR 36, 43-43 (2006).
PubMed: 16738783
Assembly members:
first cysteine catalytic half-domain, polymer, 111 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
first cysteine catalytic half-domain: FGEEMVLTDSNGEQPLSAMV
SMVTKDNPGVVTCLDEARHG
FETGDFVSFSEVQGMIQLNG
CQPMEIKVLGPYTFSICDTS
NFSDYIRGGIVSQVKVPKKI
SFKSLPASLVE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 437 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 675 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mouse E1 protein | 1 |
Entities:
Entity 1, mouse E1 protein 111 residues - Formula weight is not available
| 1 | PHE | GLY | GLU | GLU | MET | VAL | LEU | THR | ASP | SER | ||||
| 2 | ASN | GLY | GLU | GLN | PRO | LEU | SER | ALA | MET | VAL | ||||
| 3 | SER | MET | VAL | THR | LYS | ASP | ASN | PRO | GLY | VAL | ||||
| 4 | VAL | THR | CYS | LEU | ASP | GLU | ALA | ARG | HIS | GLY | ||||
| 5 | PHE | GLU | THR | GLY | ASP | PHE | VAL | SER | PHE | SER | ||||
| 6 | GLU | VAL | GLN | GLY | MET | ILE | GLN | LEU | ASN | GLY | ||||
| 7 | CYS | GLN | PRO | MET | GLU | ILE | LYS | VAL | LEU | GLY | ||||
| 8 | PRO | TYR | THR | PHE | SER | ILE | CYS | ASP | THR | SER | ||||
| 9 | ASN | PHE | SER | ASP | TYR | ILE | ARG | GLY | GLY | ILE | ||||
| 10 | VAL | SER | GLN | VAL | LYS | VAL | PRO | LYS | LYS | ILE | ||||
| 11 | SER | PHE | LYS | SER | LEU | PRO | ALA | SER | LEU | VAL | ||||
| 12 | GLU |
Samples:
sample_1: first cysteine catalytic half-domain 1.0
conditions_1: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HBHA(CO)NH | sample_1 | not available | conditions_1 |
| HNHA | sample_1 | not available | conditions_1 |
| C(CO)NH | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| NOESY-HSQC 15N-edited | sample_1 | not available | conditions_1 |
| NOESY-HSQC 13C-edited | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity+ 500 MHz
Related Database Links:
| BMRB | 18758 |
| PDB | |
| DBJ | BAA01433 BAC40121 BAC40405 BAE25369 BAE42599 |
| GB | AAH58630 AAH85791 AAI38201 AAI45985 EDL00743 |
| REF | NP_001014102 NP_001129557 NP_001263245 NP_001263246 NP_033483 |
| SP | Q02053 Q5U300 |
| AlphaFold | Q02053 Q5U300 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks