BMRB Entry 7049

Title:
1H chemical shifts of the fifth transmembrane segment of Na,K-ATPase in SDS micelles
Deposition date:
2006-03-31
Original release date:
2006-08-31
Authors:
Underhaug, Jarl; Jakobsen, Louise; Fojan, Peter; Esmann, Mikael; Malmendal, Anders; Nielsen, Niels Chr.
Citation:

Citation: Underhaug, Jarl; Jakobsen, Louise; Esmann, Mikael; Malmendal, Anders; Nielsen, Niels Chr.. "NMR studies of the fifth transmembrane segment of Na(+),K(+)-ATPase reveals a non-helical ion-binding region."  FEBS Lett. 580, 4777-4783 (2006).
PubMed: 16904671

Assembly members:

Assembly members:
M5 peptide from Na,K-ATPase, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo Sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M5 peptide from Na,K-ATPase: KKSIAYTLTSNIPEITPFLI FIIANIPL

Data sets:
Data typeCount
15N chemical shifts4
1H chemical shifts209

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1M51

Entities:

Entity 1, M5 28 residues - Formula weight is not available

1   LYSLYSSERILEALATYRTHRLEUTHRSER
2   ASNILEPROGLUILETHRPROPHELEUILE
3   PHEILEILEALAASNILEPROLEU

Samples:

2.5mM: M5 peptide from Na,K-ATPase, [15N]-ALA5,LEU8,LEU19,ALA24, 2.5 mM; SDS-d25 400 mM; NaCl 100 mM; Sodium phosphate 20 mM

5.0mM: M5 peptide from Na,K-ATPase, [15N]-ALA5,LEU8,LEU19,ALA24, 5.0 mM; SDS-d25 400 mM; NaCl 100 mM; Sodium phosphate 20 mM

conditions_1: pH: 7.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSY5.0mMnot availableconditions_1
TOCSY2.5mMnot availableconditions_1
NOESY2.5mMnot availableconditions_1
HSQC-NOESY2.5mMnot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 400.13 MHz
  • Varian Inova 799.81 MHz

Related Database Links:

PDB
DBJ BAA00061 BAE28712 BAE29635 BAE34957 BAG10675
EMBL CAA26581 CAA26582 CAA27576 CAA27840 CAA29306
GB AAA19022 AAA31002 AAA40775 AAA41671 AAA48607
PRF 1109241A 1208322A 1210234A 1309271A 1513185A
REF NP_000692 NP_001003306 NP_001009360 NP_001070266 NP_001082580
SP P04074 P05023 P05024 P06685 P09572
TPG DAA31562
AlphaFold P04074 P05023 P05024 P09572 P06685

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks