BMRB Entry 7049

Name: 1H chemical shifts of the fifth transmembrane segment of Na,K-ATPase in SDS micelles
Published: 2006-08-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7049

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H chemical shifts of the fifth transmembrane segment of Na,K-ATPase in SDS micelles

Deposition date:

2006-03-31

Original release date:

2006-08-31

Authors:

Underhaug, Jarl; Jakobsen, Louise; Fojan, Peter; Esmann, Mikael; Malmendal, Anders; Nielsen, Niels Chr.

Citation:

Citation: Underhaug, Jarl; Jakobsen, Louise; Esmann, Mikael; Malmendal, Anders; Nielsen, Niels Chr.. "NMR studies of the fifth transmembrane segment of Na(+),K(+)-ATPase reveals a non-helical ion-binding region." FEBS Lett. 580, 4777-4783 (2006).
PubMed: 16904671

Assembly members:

Assembly members:
M5 peptide from Na,K-ATPase, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M5 peptide from Na,K-ATPase: KKSIAYTLTSNIPEITPFLI FIIANIPL

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
15N chemical shifts	4
1H chemical shifts	209

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	M5	1

Entities:

Entity 1, M5 28 residues - Formula weight is not available

1

LYS

SER

ILE

ALA

TYR

THR

LEU

THR

SER

2

ASN

ILE

PRO

GLU

ILE

THR

PRO

PHE

LEU

ILE

3

PHE

ILE

ALA

ASN

ILE

PRO

LEU

Samples:

2.5mM: M5 peptide from Na,K-ATPase, [15N]-ALA5,LEU8,LEU19,ALA24, 2.5 mM; SDS-d25 400 mM; NaCl 100 mM; Sodium phosphate 20 mM

5.0mM: M5 peptide from Na,K-ATPase, [15N]-ALA5,LEU8,LEU19,ALA24, 5.0 mM; SDS-d25 400 mM; NaCl 100 mM; Sodium phosphate 20 mM

conditions_1: pH: 7.0; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
DQF-COSY	5.0mM	not available	conditions_1
TOCSY	2.5mM	not available	conditions_1
NOESY	2.5mM	not available	conditions_1
HSQC-NOESY	2.5mM	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 400.13 MHz
Varian Inova 799.81 MHz

PDB	3B8E 3KDP 3N23 3N2F 3WGU 3WGV 4HQJ 4HYT
DBJ	BAA00061 BAE28712 BAE29635 BAE34957 BAG10675
EMBL	CAA26581 CAA26582 CAA27576 CAA27840 CAA29306
GB	AAA19022 AAA31002 AAA40775 AAA41671 AAA48607
PRF	1109241A 1208322A 1210234A 1309271A 1513185A
REF	NP_000692 NP_001003306 NP_001009360 NP_001070266 NP_001082580
SP	P04074 P05023 P05024 P06685 P09572
TPG	DAA31562
AlphaFold	P04074 P05023 P05024 P06685 P09572