BMRB Entry 7034

Title:
1H, 13C, and 15N chemical shift assignments for the zinc-finger region of human ZHX1
Deposition date:
2006-03-27
Original release date:
2006-04-05
Authors:
Wienk, Hans; Lammers, Ivonne; Wu, Jin; Hotze, Anna; Wechselberger, Rainer; Kaptein, Rob; Folkers, Gert
Citation:

Citation: Wienk, Hans; Lammers, Ivonne; Hotze, Anna; Wu, Jin; Wechselberger, Rainer; Owens, Ray; Stammers, David; Stuart, David; Kaptein, Robert; Folkers, Gert. "The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension."  Biochemistry 48, 4431-4439 (2009).
PubMed: 19348505

Assembly members:

Assembly members:
hZHX1-ZNF, polymer, 102 residues, 12110 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts309
15N chemical shifts86
1H chemical shifts532

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1polypeptide1
2zinc12
3zinc22

Entities:

Entity 1, polypeptide 102 residues - 12110 Da.

1   METALAHISHISHISHISHISHISASNGLN
2   GLNASNLYSLYSVALGLUGLYGLYTYRGLU
3   CYSLYSTYRCYSTHRPHEGLNTHRPROASP
4   LEUASNMETPHETHRPHEHISVALASPSER
5   GLUHISPROASNVALVALLEUASNSERSER
6   TYRVALCYSVALGLUCYSASNPHELEUTHR
7   LYSARGTYRASPALALEUSERGLUHISASN
8   LEULYSTYRHISPROGLYGLUGLUASNPHE
9   LYSLEUTHRMETVALLYSARGASNASNGLN
10   THRILEPHEGLUGLNTHRILEASNASPLEU
11   THRPHE

Entity 2, zinc1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: hZHX1-ZNF, [U-98% 13C; U-99% 15N], 0.3 mM; NaPO4 30 mM; NaCl 100 mM; ZnCl2 50 microM; D2O 10%

conditions_1: pH: 8.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)HAsample_1not availableconditions_1
CC(CO)NHsample_1not availableconditions_1
H(C)CH-TOCSYsample_1not availableconditions_1
(H)CCH-COSYsample_1not availableconditions_1
HNH-NOESYsample_1not availableconditions_1
HH-NOESYsample_1not availableconditions_1

Software:

SPARKY v3, T. D. Goddard and D. G. Kneller - assignment

XWIN-NMR v3.5, BRUKER - processing

NMR spectrometers:

  • Bruker Avance 700.13 MHz
  • Bruker Avance 900.21 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks