BMRB Entry 7023

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7023

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Title:
Assignment of 1H,13C and 15N resonances for the REF2-I mRNA export factor
Deposition date:
2006-03-16
Original release date:
2006-06-26
Authors:
Golovanov, Alexander; Hautbergue, Guillaume; Wilson, Stuart; Lian, Lu-Yun
Citation:

Citation: Golovanov, Alexander; Hautbergue, Guillaume; Wilson, Stuart; Lian, Lu-Yun. "Assignment of 1H, 13C, and 15N resonances for the REF2-I mRNA export factor"  J. Biomol. NMR 36, 41-41 (2006).
PubMed: 16738786

Assembly members:

Assembly members:
REF2-I residues 1-155, polymer, 177 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: PET24B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
REF2-I residues 1-155: MASMTGGQQMGRDPMADKMD MSLDDIIKLNRNQRRVNRGG GPRRNRPAIARGGRNRPAPY SRPKPLPDKWQHDLFDSGCG GGEGVETGAKLLVSNLDFGV SDADIQELFAEFGTLKKAAV DYDRSGRSLGTADVHFERRA DALKAMKQYKGVPLDGRPMD IQLVASQIDLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts576
15N chemical shifts171
1H chemical shifts1068

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chain 11

Entities:

Entity 1, chain 1 177 residues - Formula weight is not available

14-residue N-terminal T7 tag (MASMTGGQQMGRDP) was added to the sequence of protein. C-terminal His6-tag with linker region is also added (LEHHHHHH); signals from five out of six histidins are invisible in the spectra and hence are not assigned.

1   METALASERMETTHRGLYGLYGLNGLNMET
2   GLYARGASPPROMETALAASPLYSMETASP
3   METSERLEUASPASPILEILELYSLEUASN
4   ARGASNGLNARGARGVALASNARGGLYGLY
5   GLYPROARGARGASNARGPROALAILEALA
6   ARGGLYGLYARGASNARGPROALAPROTYR
7   SERARGPROLYSPROLEUPROASPLYSTRP
8   GLNHISASPLEUPHEASPSERGLYCYSGLY
9   GLYGLYGLUGLYVALGLUTHRGLYALALYS
10   LEULEUVALSERASNLEUASPPHEGLYVAL
11   SERASPALAASPILEGLNGLULEUPHEALA
12   GLUPHEGLYTHRLEULYSLYSALAALAVAL
13   ASPTYRASPARGSERGLYARGSERLEUGLY
14   THRALAASPVALHISPHEGLUARGARGALA
15   ASPALALEULYSALAMETLYSGLNTYRLYS
16   GLYVALPROLEUASPGLYARGPROMETASP
17   ILEGLNLEUVALALASERGLNILEASPLEU
18   GLUHISHISHISHISHISHIS

Samples:

sample_1: REF2-I residues 1-155, [U-13C; U-15N], 0.5 – 1.0 mM; L-Arg 50 mM; L-Glu 50 mM; NaCl 100 mM; 2-mercaptoethanol 50 mM; DTT 10 mM; EDTA 5 mM; Na phosphate 20 mM; H2O 95%; D2O 5%

sample_2: REF2-I residues 1-155, [U-15N], 0.5 – 1.0 mM; L-Arg 50 mM; L-Glu 50 mM; NaCl 100 mM; 2-mercaptoethanol 50 mM; DTT 10 mM; EDTA 5 mM; Na phosphate 20 mM; D2O 100%

conditions_1: pH: 6.3; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
1H13C_HSQCnot availablenot availableconditions_1
3D HNCOnot availablenot availableconditions_1
3D HN(CA)COnot availablenot availableconditions_1
3D HNCAnot availablenot availableconditions_1
3D HN(CO)CAnot availablenot availableconditions_1
3D CBCA(CO)NHnot availablenot availableconditions_1
3D CBCANHnot availablenot availableconditions_1
3D HBHA(CO)NHnot availablenot availableconditions_1
13C-HCCH-TOCSYnot availablenot availableconditions_1
15N-TOCSY-HSQCnot availablenot availableconditions_1
15N-NOESY-HSQCnot availablenot availableconditions_1
2D TOCSYnot availablenot availableconditions_1
2D NOESYnot availablenot availableconditions_1

Software:

xwinnmr v3.5, Bruker -

NMR spectrometers:

  • Bruker DRX600 600 MHz

Related Database Links:

PDB
DBJ BAC33282
EMBL CAB76384
GB AAH99405 ADJ67482 EDL39077
REF NP_062357
SP Q9JJW6
AlphaFold Q9JJW6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks