BMRB Entry 7021

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7021

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone dynamics and domain motions of the Mip protein from legionella pneumophilia in solution
Deposition date:
2006-03-15
Original release date:
2011-08-11
Authors:
Horstmann, Martin; Ehses, Philipp; Schweimer, Kristian; Kamphausen, Thilo; Fischer, Gunter; Steinert, Michael; Hacker, org; osch, Paul; Faber, Cornelius
Citation:

Citation: Horstmann, Martin; Ehses, Philipp; Schweimer, Kristian; Steinert, Michael; Kamphausen, Thilo; Fischer, Gunter; Hacker, Jorg; Roesch, Paul; Faber, Cornelius. "Domain Motions of the Mip Protein from Legionella pneumophila"  Biochemistry 45, 12303-12311 (2006).
PubMed: 17014083

Assembly members:

Assembly members:
LpMip, polymer, 213 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Legionella pneumophila   Taxonomy ID: 446   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Legionella L. pneumophila

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LpMip: ATDATSLATDKDKLSYSIGA DLGKNFKNQGIDVNPEAMAK GMQDAMSGAQLALTEQQMKD VLNKFQKDLMAKRTAEFNKK ADENKVKGEAFLTENKNKPG VVVLPSGLQYKVINSGNGVK PGKSDTVTVEYTGRLIDGTV FDSTEKTGKPATFQVSQVIP GWTEALQLMPAGSTWEIYVP SGLAYGPRSVGGPIGPNETL IFKIHLISVKKSS

Data sets:
Data typeCount
13C chemical shifts663
15N chemical shifts178
1H chemical shifts969
T1 relaxation values161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LpMip is a homodimer1
2LpMip is a homodimer1

Entities:

Entity 1, LpMip is a homodimer 213 residues - Formula weight is not available

1   ALATHRASPALATHRSERLEUALATHRASP
2   LYSASPLYSLEUSERTYRSERILEGLYALA
3   ASPLEUGLYLYSASNPHELYSASNGLNGLY
4   ILEASPVALASNPROGLUALAMETALALYS
5   GLYMETGLNASPALAMETSERGLYALAGLN
6   LEUALALEUTHRGLUGLNGLNMETLYSASP
7   VALLEUASNLYSPHEGLNLYSASPLEUMET
8   ALALYSARGTHRALAGLUPHEASNLYSLYS
9   ALAASPGLUASNLYSVALLYSGLYGLUALA
10   PHELEUTHRGLUASNLYSASNLYSPROGLY
11   VALVALVALLEUPROSERGLYLEUGLNTYR
12   LYSVALILEASNSERGLYASNGLYVALLYS
13   PROGLYLYSSERASPTHRVALTHRVALGLU
14   TYRTHRGLYARGLEUILEASPGLYTHRVAL
15   PHEASPSERTHRGLULYSTHRGLYLYSPRO
16   ALATHRPHEGLNVALSERGLNVALILEPRO
17   GLYTRPTHRGLUALALEUGLNLEUMETPRO
18   ALAGLYSERTHRTRPGLUILETYRVALPRO
19   SERGLYLEUALATYRGLYPROARGSERVAL
20   GLYGLYPROILEGLYPROASNGLUTHRLEU
21   ILEPHELYSILEHISLEUILESERVALLYS
22   LYSSERSER

Samples:

sample_1: LpMip, [U-13C; U-15N], 0.9 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availablenot available
CBCA(CO)NHsample_1not availablenot available
HNCOsample_1not availablenot available
HNCACBsample_1not availablenot available
HNCAsample_1not availablenot available
HBHA(CO)NHsample_1not availablenot available
C(CO)NHsample_1not availablenot available
HCCHTOCSYsample_1not availablenot available
15N NOESYsample_1not availablenot available
13C NOESYsample_1not availablenot available
15N 15N NOESYsample_1not availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks