BMRB Entry 7019

Title:
NMR assignment of the protein nsp3a from SARS-CoV
Deposition date:
2006-03-10
Original release date:
2007-02-14
Authors:
Serrano, Pedro; Almeida, Marcius; Johnson, Margaret; Saikatendu, Kumar; Subramanian, Vanita; Joseph, Jeremiah; Stevens, Raymond; Kuhn, Peter; Wuthrich, Kurt
Citation:

Citation: Serrano, Pedro; Almeida, Marcius; Johnson, Margaret; Wuthrich, Kurt. "NMR assignment of the protein nsp3a from SARS-CoV"  J. Biomol. NMR 36, 45-45 (2006).
PubMed: 16799860

Assembly members:

Assembly members:
nsp3a, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: Coronavirus SARS

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts496
15N chemical shifts117
1H chemical shifts773

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp3a1

Entities:

Entity 1, nsp3a 112 residues - Formula weight is not available

1   ALAPROILELYSGLYVALTHRPHEGLYGLU
2   ASPTHRVALTRPGLUVALGLNGLYTYRLYS
3   ASNVALARGILETHRPHEGLULEUASPGLU
4   ARGVALASPLYSVALLEUASNGLULYSCYS
5   SERVALTYRTHRVALGLUSERGLYTHRGLU
6   VALTHRGLUPHEALACYSVALVALALAGLU
7   ALAVALVALLYSTHRLEUGLNPROVALSER
8   ASPLEULEUTHRASNMETGLYILEASPLEU
9   ASPGLUTRPSERVALALATHRPHETYRLEU
10   PHEASPASPALAGLYGLUGLUASNPHESER
11   SERARGMETTYRCYSSERPHETYRPROPRO
12   ASPGLU

Samples:

sample_1: nsp3a, [U-13C; U-15N], 1 – 2.5 mM; sodium phosphate 50 mM; NaCl 150 mM

conditions_1: pH: 6.5

Experiments:

NameSampleSample stateSample conditions
15N H-HSQCsample_1not availableconditions_1
13C H-HSQCsample_1not availableconditions_1
3D HNCACBsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
3D CACBCONHsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D HCCH-TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 700 MHz

Related Database Links:

PDB
DBJ BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF NP_828849 NP_828850 NP_828862
SP P0C6U8 P0C6X7
AlphaFold P0C6U8 P0C6X7 P0C6U8 P0C6X7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks