BMRB Entry 7015

Name: NMR assignments for apo- and Ca2+ saturated paramecium calmodulin
Published: 2013-02-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7015

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignments for apo- and Ca2+ saturated paramecium calmodulin

Deposition date:

2005-06-24

Original release date:

2013-02-15

Authors:

Jaren, Olav; Kranz, James; Sorensen, Brenda; Wand, A.; Shea, Madeline

Citation:

Citation: Jaren, Olav; Kranz, James; Sorensen, Brenda; Wand, A.; Shea, Madeline. "Calcium-induced conformational switching of Paramecium calmodulin provides evidence for domain coupling." Biochemistry 41, 14158-14166 (2002).
PubMed: 12450379

Assembly members:

Assembly members:
PCaM under apo conditions, polymer, 148 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Paramecium Taxonomy ID: 5884 Superkingdom: Eukaryota Kingdom: Protista Genus/species: Paramecium not available

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PCaM under apo conditions: AEQLTEEQIAEFKEAFALFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLSLMARKMKEQD SEEELIEAFKVFDRDGNGLI SAAELRHVMTNLGEKLTDDE VDEMIREADIDGDGHINYEE FVRMMVSK

Data sets:

assigned_chemical_shifts
- chem_shift_PCaM

Data type	Count
13C chemical shifts	402
15N chemical shifts	144
1H chemical shifts	144

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	paramecium calmodulin	1
2	Calcium ion	2

Entities:

Entity 1, paramecium calmodulin 148 residues - Formula weight is not available

1

ALA

GLU

GLN

LEU

THR

GLU

GLN

ILE

ALA

2

GLU

PHE

LYS

GLU

ALA

PHE

ALA

LEU

PHE

ASP

3

LYS

ASP

GLY

ASP

GLY

THR

ILE

THR

LYS

4

GLU

LEU

GLY

THR

VAL

MET

ARG

SER

LEU

GLY

5

GLN

ASN

PRO

THR

GLU

ALA

GLU

LEU

GLN

ASP

6

MET

ILE

ASN

GLU

VAL

ASP

ALA

ASP

GLY

ASN

7

GLY

THR

ILE

ASP

PHE

PRO

GLU

PHE

LEU

SER

8

LEU

MET

ALA

ARG

LYS

MET

LYS

GLU

GLN

ASP

9

SER

GLU

LEU

ILE

GLU

ALA

PHE

LYS

10

VAL

PHE

ASP

ARG

ASP

GLY

ASN

GLY

LEU

ILE

11

SER

ALA

GLU

LEU

ARG

HIS

VAL

MET

THR

12

ASN

LEU

GLY

GLU

LYS

LEU

THR

ASP

GLU

13

VAL

ASP

GLU

MET

ILE

ARG

GLU

ALA

ASP

ILE

14

ASP

GLY

ASP

GLY

HIS

ILE

ASN

TYR

GLU

15

PHE

VAL

ARG

MET

VAL

SER

LYS

Entity 2, Calcium ion - Ca - 40.078 Da.

1

CA

Samples:

sample_1: PCaM, [U-13C; U-15N], 1.25 mM; imidazole-d4 10 mM; KCl 100 mM; CaCl2 5 mM; NaN3 0.01%; D2O 8%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

FELIX, Molecular Simulations Inc. - spectra processing

NMRView - processed spectra examination

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz
Varian INOVA 700 MHz

BMRB	6707
PDB	1CLM 1EXR 1N0Y 1OSA
EMBL	CAK80966
GB	AAA29443 AAB20487 AAB31200 AAT38517
REF	XP_001448363
SP	P07463
AlphaFold	P07463