BMRB Entry 7013

Name: 1H, 15N, 13C resonance assignments for Bcl-xL protein 38 kDa dimer
Published: 2006-03-31

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7013

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C resonance assignments for Bcl-xL protein 38 kDa dimer

Deposition date:

2006-03-01

Original release date:

2006-03-31

Authors:

Denisov, Alexey; Gehring, Kalle

Citation:

Citation: Denisov, Alexey; Sprules, Tara; Fraser, James; Kozlov, Guennadi; Gehring, Kalle. "Heat-induced dimerization of Bcl-xL through alpha-helix swapping" Biochemistry 46, 734-740 (2007).
PubMed: 17223694

Assembly members:

Assembly members:
Bcl-xL, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET-29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bcl-xL: SQSNRELVVDFLSYKLSQRG YSWSQFSDVEENRTEAPEET EAEAVKQALREAGDEFELRY RRAFSDLTSQLHITPGTAYQ SFEQVVNELFRDGVNWGRIV AFFSFGGALCVESVDKEMQV LVSRIASWMATYLNDHLEPW IQENGGWDTFVDLYGLEHHH HHH

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	432
15N chemical shifts	151
1H chemical shifts	208

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bcl-xL, chain 1	1
2	Bcl-xL, chain 2	1

Entities:

Entity 1, Bcl-xL, chain 1 163 residues - Formula weight is not available

Protein contains C-terminal His-tag LEHHHHHH

1

SER

GLN

SER

ASN

ARG

GLU

LEU

VAL

ASP

2

PHE

LEU

SER

TYR

LYS

LEU

SER

GLN

ARG

GLY

3

TYR

SER

TRP

SER

GLN

PHE

SER

ASP

VAL

GLU

4

GLU

ASN

ARG

THR

GLU

ALA

PRO

GLU

THR

5

GLU

ALA

GLU

ALA

VAL

LYS

GLN

ALA

LEU

ARG

6

GLU

ALA

GLY

ASP

GLU

PHE

GLU

LEU

ARG

TYR

7

ARG

ALA

PHE

SER

ASP

LEU

THR

SER

GLN

8

LEU

HIS

ILE

THR

PRO

GLY

THR

ALA

TYR

GLN

9

SER

PHE

GLU

GLN

VAL

ASN

GLU

LEU

PHE

10

ARG

ASP

GLY

VAL

ASN

TRP

GLY

ARG

ILE

VAL

11

ALA

PHE

SER

PHE

GLY

ALA

LEU

CYS

12

VAL

GLU

SER

VAL

ASP

LYS

GLU

MET

GLN

VAL

13

LEU

VAL

SER

ARG

ILE

ALA

SER

TRP

MET

ALA

14

THR

TYR

LEU

ASN

ASP

HIS

LEU

GLU

PRO

TRP

15

ILE

GLN

GLU

ASN

GLY

TRP

ASP

THR

PHE

16

VAL

ASP

LEU

TYR

GLY

LEU

GLU

HIS

17

HIS

Samples:

sample_1: Bcl-xL dimer, [U-99% 13C; U-99% 15N; U-80% 2D], 1 mM; phosphate buffer 25 mM; D2O 10%; H2O 90%

conditions_1: pH: 7.0; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N-TROSY-HSQC	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
3D-NOESY-HSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1

Software:

XEASY v1.3.13 - processing

NMR spectrometers:

Varian Unity Inova 800 MHz