BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7002

Title: 1H, 13C, and 15N Chemical Shift Assignments for the Ede1 UBA-ubiquitin complex   PubMed: 16563434

Deposition date: 2006-02-22 Original release date: 2006-04-27

Authors: Swanson, Kurt; Radhakrishnan, Ishwar

Citation: Swanson, Kurt; Hicke, Linda; Radhakrishnan, Ishwar. "Structural Basis for Monoubiquitin Recognition by the Ede1 UBA Domain"  J. Mol. Biol. 358, 713-724 (2006).

Assembly members:
Ubiquitin, polymer, 76 residues, Formula weight is not available
Ede1 UBA, polymer, 43 residues, Formula weight is not available

Natural source:   Common Name: Yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Ubiquitin: MQIFVKTLTGKTITLEVESS DTIDNVKSKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG
Ede1 UBA: TTPKSLAVEELSGMGFTEEE AHNALEKCNWDLEAATNFLL DSA

Data sets:
Data typeCount
13C chemical shifts500
15N chemical shifts124
1H chemical shifts805

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubiquitin1
2Ede1 UBA2

Entities:

Entity 1, Ubiquitin 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUSERSER
3   ASPTHRILEASPASNVALLYSSERLYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Entity 2, Ede1 UBA 43 residues - Formula weight is not available

1   THRTHRPROLYSSERLEUALAVALGLUGLU
2   LEUSERGLYMETGLYPHETHRGLUGLUGLU
3   ALAHISASNALALEUGLULYSCYSASNTRP
4   ASPLEUGLUALAALATHRASNPHELEULEU
5   ASPSERALA

Samples:

sample_1: Ubiquitin 1 mM; Ede1 UBA 1 mM; Sodium Phosphate 20 mM

conditions_1: pH: 6.0; temperature: . .

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    PDB
    DBJ GAA21503
    EMBL CAA80797 CAA84867 CAY77738
    GB AHY74439 EDN64569 EDV12138 EDZ73911 EEU07701
    REF NP_009506 XP_011102417
    SP P34216
    TPG DAA07071

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