BMRB Entry 6998

Title:
Phosphorylation of Cytoplasmic Tail of Tissue Factor and its Role in Modulating Structure and Binding Affinity.   PubMed: 20076769
Deposition date:
2006-02-21
Original release date:
2007-06-20
Authors:
Sen, Mehmet; Agrawal, Sameer; Craft, John; Ruf, Wolfram; Legge, Glen
Citation:

Citation: Sen, Mehmet; Herzik, Mark; Craft, John; Creath, Andrea; Agrawal, Sameer; Ruf, Wolfram; Legge, Glen. "Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region"  Open Spectrosc J. 3, 58-64 (2009).

Assembly members:

Assembly members:
TFSP258, polymer, 19 residues, 2218.3 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TFSP258: CRKAGVGQSWKENXPLNVS

Data sets:
Data typeCount
13C chemical shifts43
15N chemical shifts20
1H chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TFSP258, trans conformer1
2TFSP258, cis conformer1

Entities:

Entity 1, TFSP258, trans conformer 19 residues - 2218.3 Da.

1   CYSARGLYSALAGLYVALGLYGLNSERTRP
2   LYSGLUASNSEPPROLEUASNVALSER

Samples:

sample_1: TFSP258 6 ± 0.05 mM; D2O 10%

conditions_of_TFSP258: ionic strength: 0 mM; pH: 6.0; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1not availableconditions_of_TFSP258
IP-COSYsample_1not availableconditions_of_TFSP258
ROESYsample_1not availableconditions_of_TFSP258
1H1H_NOESYsample_1not availableconditions_of_TFSP258
1H15N_HSQCsample_1not availableconditions_of_TFSP258
1H13C_HSQCsample_1not availableconditions_of_TFSP258

Software:

NMRView - NMRView was used for Assignment

NMRPipe - process the Raw NMR data

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts