BMRB Entry 6991

Title:
Phosphorylation of Cytoplasmic Tail of Tissue Factor and its Role in Modulating
Deposition date:
2006-02-15
Original release date:
2007-06-20
Authors:
Sen, Mehmet; Agrawal, Sameer; Craft, John; Ruf, Wolfram; Legge, Glen
Citation:

Citation: Sen, Mehmet; Herzik, Mark; Craft, John; Creath, Andrea; Agrawal, Sameer; Ruf, Wolfram; Legge, Glen. "Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region"  Open Spectrosc J. 3, 58-64 (2009).
PubMed: 20076769

Assembly members:

Assembly members:
TFCD, polymer, 19 residues, 2060.3 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TFCD: CRKAGVGQSWKENSPLNVS

Data sets:
Data typeCount
13C chemical shifts39
15N chemical shifts19
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TFCD, trans conformer1
2TFCD, cis conformer1

Entities:

Entity 1, TFCD, trans conformer 19 residues - 2060.3 Da.

1   CYSARGLYSALAGLYVALGLYGLNSERTRP
2   LYSGLUASNSERPROLEUASNVALSER

Samples:

sample_1: TFCD 6 ± 0.05 mM; D2O 10%

conditions_of_TFCD: ionic strength: 0 mM; pH: 6.0; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1not availableconditions_of_TFCD
IP-COSYsample_1not availableconditions_of_TFCD
ROESYsample_1not availableconditions_of_TFCD
1H1H_NOESYsample_1not availableconditions_of_TFCD
1H15N_HSQCsample_1not availableconditions_of_TFCD
1H13C_HSQCsample_1not availableconditions_of_TFCD

Software:

NMRView - NMRView was used for Assignment

NMRPipe - NMR data Processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks