BMRB Entry 6985

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the human Hexim1 TBD
Published: 2006-09-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6985

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the human Hexim1 TBD

Deposition date:

2006-02-14

Original release date:

2006-09-07

Authors:

Dames, Sonja; Schoenichen, Andre; Grzesiek, Stephan; Geyer, Matthias

Citation:

Citation: Dames, Sonja; Schoenichen, Andre; Grzesiek, Stephan; Geyer, Matthias. "NMR assignment of the Cyclin T-binding domain of human Hexim 1" J. Biomol. NMR 36, 39-39 (2006).
PubMed: 16917728

Assembly members:

Assembly members:
Hexim1 TBD, polymer, 107 residues, 12661 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Vector: Novagen

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hexim1 TBD: GAMAGDGSEFLQRDFSETYE RYHTESLQNMSKQELIKEYL ELEKCLSRMEDENNRLRLES KRLGGDDARVRELELELDRL RAENLQLLTENELHRQQERA PLSKFGD

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	447
15N chemical shifts	112
1H chemical shifts	740

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1
2	subunit 2	1

Entities:

Entity 1, subunit 1 107 residues - 12661 Da.

cloning artefact: N-terminal GA mutation A4 (= A256 in full length) instead of G

1

GLY

ALA

MET

ALA

GLY

ASP

GLY

SER

GLU

PHE

2

LEU

GLN

ARG

ASP

PHE

SER

GLU

THR

TYR

GLU

3

ARG

TYR

HIS

THR

GLU

SER

LEU

GLN

ASN

MET

4

SER

LYS

GLN

GLU

LEU

ILE

LYS

GLU

TYR

LEU

5

GLU

LEU

GLU

LYS

CYS

LEU

SER

ARG

MET

GLU

6

ASP

GLU

ASN

ARG

LEU

ARG

LEU

GLU

SER

7

LYS

ARG

LEU

GLY

ASP

ALA

ARG

VAL

8

ARG

GLU

LEU

GLU

LEU

GLU

LEU

ASP

ARG

LEU

9

ARG

ALA

GLU

ASN

LEU

GLN

LEU

THR

GLU

10

ASN

GLU

LEU

HIS

ARG

GLN

GLU

ARG

ALA

11

PRO

LEU

SER

LYS

PHE

GLY

ASP

Samples:

sample_1: Hexim1 TBD, [U-90% 15N], 1.2 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; H2O 95%; D2O 5%

sample_2: Hexim1 TBD, [U-95% 13C; U-90% 15N], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; H2O 95%; D2O 5%

sample_3: Hexim1 TBD, [U-95% 13C; U-90% 15N], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; D2O 100%

sample_4: Hexim1 TBD, [U-10% 13C], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; D2O 100%

sample_5: Hexim1 TBD, [U-95% 13C; U-90% 15N], 1 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; H2O 95%; D2O 5%

sample_6: Hexim1 TBD, [U-95% 13C; U-90% 15N], 0.6 mM; potassium phosphate 20 mM; sodium chloride 50 mM; TCEP-HCl 10 mM; DTE 1 mM; PF1 phage 16 mg/ml; H2O 95%; D2O 5%

conditions_1: pH: 7.2; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

NMRPipe, F. Delaglio - data processing

NMRView v5.0.4, B. Johnson - resonance assignment

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz

PDB	2GD7
DBJ	BAA36166 BAG36332 BAG51211 BAG74079
GB	AAH06460 ABM84198 ABM87157 ABM87602 AIC55820
REF	NP_001181605 NP_006451 XP_001488620 XP_002919799 XP_003270766
SP	O94992
AlphaFold	O94992