BMRB Entry 6981

Title:
Solution Structure of Monomeric BsaL, the Type III Secretion Needle Protein of Burkholderia pseudomallei   PubMed: 16631790
Deposition date:
2006-02-13
Original release date:
2007-02-07
Authors:
Zhang, Lingling; Wang, Yu; Picking, Wendy; Picking, William; De Guzman, Roberto
Citation:

Citation: Zhang, Lingling; Wang, Yu; Picking, Wendy; Picking, William; De Guzman, Roberto. "Solution Structure of Monomeric BsaL, the Type III Secretion Needle Protein of Burkholderia pseudomallei"  J. Mol. Biol. 359, 322-330 (2006).

Assembly members:

Assembly members:
BsaL, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Burkholderia pseudomallei   Taxonomy ID: 28450   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia pseudomallei

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: Burkholderia pseudomallei   Taxonomy ID: 28450   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia pseudomallei

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts284
15N chemical shifts85
1H chemical shifts479

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BsaL1

Entities:

Entity 1, BsaL 92 residues - Formula weight is not available

1   METSERASNPROPROTHRPROLEULEUALA
2   ASPTYRGLUTRPSERGLYTYRLEUTHRGLY
3   ILEGLYARGALAPHEASPASPGLYVALLYS
4   ASPLEUASNLYSGLNLEUGLNASPALAGLN
5   ALAASNLEUTHRLYSASNPROSERASPPRO
6   THRALALEUALAASNTYRGLNMETILEMET
7   SERGLUTYRASNLEUTYRARGASNALAGLN
8   SERSERALAVALLYSSERMETLYSASPILE
9   ASPSERSERILELEUGLUHISHISHISHIS
10   HISHIS

Samples:

sample_1: BsaL, [U-15N; U-13C], 0.8 ± 0.2 mM

sample_2: BsaL, [U-15N], 0.8 ± 0.2 mM

conditions_1: pH: 7

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    PDB

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    SPARKY: Backbone or all simulated peaks