BMRB Entry 6971

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6971
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR assignment of the b' domain of thermophilic fungal protein disulfide isomerase
Deposition date:
2006-02-03
Original release date:
2006-08-07
Authors:
Nakano, Michiko; Murakami, Chiho; Yamaguchi, Yoshiki; Sasakawa, Hiroaki; Harada, Takushi; Kurimoto, Eiji; Asami, Osamu; Kajino, Tsutomu; Kato, Koichi
Citation:

Citation: Nakano, Michiko; Murakami, Chiho; Yamaguchi, Yoshiki; Sasakawa, Hiroaki; Harada, Takushi; Kurimoto, Eiji; Asami, Osamu; Kajino, Tsutomu; Kato, Koichi. "NMR Assignments of the b' and a' Domains of Thermophilic Fungal Protein Disulfide Isomerase"  J. Biomol. NMR 36, 44-44 (2006).
PubMed: 16819589

Assembly members:

Assembly members:
PDI-b', polymer, 133 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: thermophilic fungus   Taxonomy ID: 34413   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Humicola insolens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDI-b': GPLGSPLIGEIGPETYSDYM SAGIPLAYIFAETAEERKEL SDKLKPIAEAQRGVINFGTI DAKAFGAHAGNLNLKTDKFP AFAIQEVAKNQKFPFDQEKE ITFEAIKAFVDDFVAGKIEP SIKSEPIPEKQEG

Data sets:
Data typeCount
13C chemical shifts468
15N chemical shifts128
1H chemical shifts816

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDI-b'1

Entities:

Entity 1, PDI-b' 133 residues - Formula weight is not available

1   GLYPROLEUGLYSERPROLEUILEGLYGLU
2   ILEGLYPROGLUTHRTYRSERASPTYRMET
3   SERALAGLYILEPROLEUALATYRILEPHE
4   ALAGLUTHRALAGLUGLUARGLYSGLULEU
5   SERASPLYSLEULYSPROILEALAGLUALA
6   GLNARGGLYVALILEASNPHEGLYTHRILE
7   ASPALALYSALAPHEGLYALAHISALAGLY
8   ASNLEUASNLEULYSTHRASPLYSPHEPRO
9   ALAPHEALAILEGLNGLUVALALALYSASN
10   GLNLYSPHEPROPHEASPGLNGLULYSGLU
11   ILETHRPHEGLUALAILELYSALAPHEVAL
12   ASPASPPHEVALALAGLYLYSILEGLUPRO
13   SERILELYSSERGLUPROILEPROGLULYS
14   GLNGLUGLY

Samples:

sample_1: PDI-b', [U-13C; U-15N], 1 mM

conditions_1: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H13C HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
CBCANHsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
15N-edited TOCSYsample_1not availableconditions_1
H(CC)(CO)NHsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
1H-1H TOCSYsample_1not availableconditions_1
1H-1H NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker Avance 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

PDB
GB AAC60578 AEO66019
PRF 2018168A
REF XP_003652355
SP P55059
AlphaFold P55059

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks