BMRB Entry 6944

Name: Resonance assignment for the N-terminal region of eukaryotic initiation factor 5 (eIF5)
Published: 2006-08-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6944

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignment for the N-terminal region of eukaryotic initiation factor 5 (eIF5)

Deposition date:

2006-01-20

Original release date:

2006-08-11

Authors:

Conte, Maria; Kelly, Geoff; Babon, Jeff; Proud, Christopher

Citation:

Citation: Conte, Maria; Kelly, Geoff; Babon, Jeff; Proud, Christopher. "Resonance assignment for the N-terminal region of the eukaryotic initiation factor 5 (eIF5)" J. Biomol. NMR 36, 42-42 (2006).
PubMed: 16738784

Assembly members:

Assembly members:
Protein, polymer, 170 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein: LSVNVNRSVSDQFYRYKMPR LIAKVEGKGNGIKTVIVNMV DVAKALNRPPTYPTKYFGCE LGAQTQFDVKNDRYIVNGSH EANKLQDMLDGFIKKFVLCP ECENPETDLHVNPKKQTIGN SCKACGYRGMLDTHHKLCTF ILKNPPENSDSGTGKKEKEK KNRKGKDKEN

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	567
15N chemical shifts	148
1H chemical shifts	806

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	eIF5(1-170)	1
2	Zinc ion	2

Entities:

Entity 1, eIF5(1-170) 170 residues - Formula weight is not available

1	LEU	SER	VAL	ASN	VAL	ASN	ARG	SER	VAL	SER
2	ASP	GLN	PHE	TYR	ARG	TYR	LYS	MET	PRO	ARG
3	LEU	ILE	ALA	LYS	VAL	GLU	GLY	LYS	GLY	ASN
4	GLY	ILE	LYS	THR	VAL	ILE	VAL	ASN	MET	VAL
5	ASP	VAL	ALA	LYS	ALA	LEU	ASN	ARG	PRO	PRO
6	THR	TYR	PRO	THR	LYS	TYR	PHE	GLY	CYS	GLU
7	LEU	GLY	ALA	GLN	THR	GLN	PHE	ASP	VAL	LYS
8	ASN	ASP	ARG	TYR	ILE	VAL	ASN	GLY	SER	HIS
9	GLU	ALA	ASN	LYS	LEU	GLN	ASP	MET	LEU	ASP
10	GLY	PHE	ILE	LYS	LYS	PHE	VAL	LEU	CYS	PRO
11	GLU	CYS	GLU	ASN	PRO	GLU	THR	ASP	LEU	HIS
12	VAL	ASN	PRO	LYS	LYS	GLN	THR	ILE	GLY	ASN
13	SER	CYS	LYS	ALA	CYS	GLY	TYR	ARG	GLY	MET
14	LEU	ASP	THR	HIS	HIS	LYS	LEU	CYS	THR	PHE
15	ILE	LEU	LYS	ASN	PRO	PRO	GLU	ASN	SER	ASP
16	SER	GLY	THR	GLY	LYS	LYS	GLU	LYS	GLU	LYS
17	LYS	ASN	ARG	LYS	GLY	LYS	ASP	LYS	GLU	ASN

Entity 2, Zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: eIF5(1-170), [U-13C; U-15N], 0.5 mM; sodium phosphate 20 mM; DTT 2 mM

conditions_1: pH: 6.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample_1	not available	conditions_1
HN(CO)CA	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1
15N-and 13C edited NOESY	sample_1	not available	conditions_1
HNHA	sample_1	not available	conditions_1
15N-and 13C HSQC	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Inova 600 MHz
Varian Inova 800 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	LEU	SER	VAL	ASN	VAL	ASN	ARG	SER	VAL	SER
2	ASP	GLN	PHE	TYR	ARG	TYR	LYS	MET	PRO	ARG
3	LEU	ILE	ALA	LYS	VAL	GLU	GLY	LYS	GLY	ASN
4	GLY	ILE	LYS	THR	VAL	ILE	VAL	ASN	MET	VAL
5	ASP	VAL	ALA	LYS	ALA	LEU	ASN	ARG	PRO	PRO
6	THR	TYR	PRO	THR	LYS	TYR	PHE	GLY	CYS	GLU
7	LEU	GLY	ALA	GLN	THR	GLN	PHE	ASP	VAL	LYS
8	ASN	ASP	ARG	TYR	ILE	VAL	ASN	GLY	SER	HIS
9	GLU	ALA	ASN	LYS	LEU	GLN	ASP	MET	LEU	ASP
10	GLY	PHE	ILE	LYS	LYS	PHE	VAL	LEU	CYS	PRO
11	GLU	CYS	GLU	ASN	PRO	GLU	THR	ASP	LEU	HIS
12	VAL	ASN	PRO	LYS	LYS	GLN	THR	ILE	GLY	ASN
13	SER	CYS	LYS	ALA	CYS	GLY	TYR	ARG	GLY	MET
14	LEU	ASP	THR	HIS	HIS	LYS	LEU	CYS	THR	PHE
15	ILE	LEU	LYS	ASN	PRO	PRO	GLU	ASN	SER	ASP
16	SER	GLY	THR	GLY	LYS	LYS	GLU	LYS	GLU	LYS
17	LYS	ASN	ARG	LYS	GLY	LYS	ASP	LYS	GLU	ASN

1	LEU	SER	VAL	ASN	VAL	ASN	ARG	SER	VAL	SER
2	ASP	GLN	PHE	TYR	ARG	TYR	LYS	MET	PRO	ARG
3	LEU	ILE	ALA	LYS	VAL	GLU	GLY	LYS	GLY	ASN
4	GLY	ILE	LYS	THR	VAL	ILE	VAL	ASN	MET	VAL
5	ASP	VAL	ALA	LYS	ALA	LEU	ASN	ARG	PRO	PRO
6	THR	TYR	PRO	THR	LYS	TYR	PHE	GLY	CYS	GLU
7	LEU	GLY	ALA	GLN	THR	GLN	PHE	ASP	VAL	LYS
8	ASN	ASP	ARG	TYR	ILE	VAL	ASN	GLY	SER	HIS
9	GLU	ALA	ASN	LYS	LEU	GLN	ASP	MET	LEU	ASP
10	GLY	PHE	ILE	LYS	LYS	PHE	VAL	LEU	CYS	PRO
11	GLU	CYS	GLU	ASN	PRO	GLU	THR	ASP	LEU	HIS
12	VAL	ASN	PRO	LYS	LYS	GLN	THR	ILE	GLY	ASN
13	SER	CYS	LYS	ALA	CYS	GLY	TYR	ARG	GLY	MET
14	LEU	ASP	THR	HIS	HIS	LYS	LEU	CYS	THR	PHE
15	ILE	LEU	LYS	ASN	PRO	PRO	GLU	ASN	SER	ASP
16	SER	GLY	THR	GLY	LYS	LYS	GLU	LYS	GLU	LYS
17	LYS	ASN	ARG	LYS	GLY	LYS	ASP	LYS	GLU	ASN

1	LEU	SER	VAL	ASN	VAL	ASN	ARG	SER	VAL	SER
2	ASP	GLN	PHE	TYR	ARG	TYR	LYS	MET	PRO	ARG
3	LEU	ILE	ALA	LYS	VAL	GLU	GLY	LYS	GLY	ASN
4	GLY	ILE	LYS	THR	VAL	ILE	VAL	ASN	MET	VAL
5	ASP	VAL	ALA	LYS	ALA	LEU	ASN	ARG	PRO	PRO
6	THR	TYR	PRO	THR	LYS	TYR	PHE	GLY	CYS	GLU
7	LEU	GLY	ALA	GLN	THR	GLN	PHE	ASP	VAL	LYS
8	ASN	ASP	ARG	TYR	ILE	VAL	ASN	GLY	SER	HIS
9	GLU	ALA	ASN	LYS	LEU	GLN	ASP	MET	LEU	ASP
10	GLY	PHE	ILE	LYS	LYS	PHE	VAL	LEU	CYS	PRO
11	GLU	CYS	GLU	ASN	PRO	GLU	THR	ASP	LEU	HIS
12	VAL	ASN	PRO	LYS	LYS	GLN	THR	ILE	GLY	ASN
13	SER	CYS	LYS	ALA	CYS	GLY	TYR	ARG	GLY	MET
14	LEU	ASP	THR	HIS	HIS	LYS	LEU	CYS	THR	PHE
15	ILE	LEU	LYS	ASN	PRO	PRO	GLU	ASN	SER	ASP
16	SER	GLY	THR	GLY	LYS	LYS	GLU	LYS	GLU	LYS
17	LYS	ASN	ARG	LYS	GLY	LYS	ASP	LYS	GLU	ASN