BMRB Entry 6938

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6938
MolProbity Validation Chart

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Title:
1H, 13C and 15N NMR assignment of the region 51-160 of human KIN17 protein
Deposition date:
2006-01-13
Original release date:
2006-05-02
Authors:
Carlier, Ludovic; le Maire, Albane; Braud, Sandrine; Masson, Cedric; Gondry, Muriel; Zinn-Justin, Sophie; Guilhaudis, Laure; Milazzo, Isabelle; Davoust, Daniel; Gilquin, Bernard; Couprie, Joel
Citation:

Citation: Carlier, Ludovic; le Maire, Albane; Braud, Sandrine; Masson, Cedric; Gondry, Muriel; Zinn-Justin, Sophie; Guilhaudis, Laure; Milazzo, Isabelle; Davoust, Daniel; Gilquin, Bernard; Couprie, Joel. "NMR Assignment of Region 51-160 of Human KIN17, a DNA and RNA-binding Protein"  J. Biomol. NMR 36, 29-29 (2006).
PubMed: 16636754

Assembly members:

Assembly members:
K2, polymer, 111 residues, 13600 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pEXP-TH5

Entity Sequences (FASTA):

Entity Sequences (FASTA):
K2: GQRQLLLASENPQQFMDYFS EEFRNDFLELLRRRFGTKRV HNNIVYNEYISHREHIHMNA TQWETLTDFTKWLGREGLCK VDETPKGWYIQYIDRDPETI RRQLELEKKKK

Data sets:
Data typeCount
13C chemical shifts531
15N chemical shifts120
1H chemical shifts829

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer region 51-160 of human KIN171

Entities:

Entity 1, monomer region 51-160 of human KIN17 111 residues - 13600 Da.

1   GLYGLNARGGLNLEULEULEUALASERGLU
2   ASNPROGLNGLNPHEMETASPTYRPHESER
3   GLUGLUPHEARGASNASPPHELEUGLULEU
4   LEUARGARGARGPHEGLYTHRLYSARGVAL
5   HISASNASNILEVALTYRASNGLUTYRILE
6   SERHISARGGLUHISILEHISMETASNALA
7   THRGLNTRPGLUTHRLEUTHRASPPHETHR
8   LYSTRPLEUGLYARGGLUGLYLEUCYSLYS
9   VALASPGLUTHRPROLYSGLYTRPTYRILE
10   GLNTYRILEASPARGASPPROGLUTHRILE
11   ARGARGGLNLEUGLULEUGLULYSLYSLYS
12   LYS

Samples:

sample_1: KIN17, [U-15N], 0.7 mM; NaCl 150 mM; NaH2PO4/Na2HPO4 Phosphate 50 mM; H2O 90%; D2O 10%; TCEP 1 mM

sample_2: KIN17, [U-13C; U-15N], 0.8 mM; NaCl 150 mM; NaH2PO4/Na2HPO4 Phosphate 50 mM; H2O 90%; D2O 10%; TCEP 1 mM

sample_3: KIN17, [U-13C; U-15N], 0.7 mM; NaCl 150 mM; NaH2PO4/Na2HPO4 Phosphate 50 mM; D2O 100%; TCEP 1 mM

conditions_1: ionic strength: 200 mM; pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N_HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
CBCANHnot availablenot availableconditions_1
(HCA)CO(CA)NHnot availablenot availableconditions_1
HBHACONHnot availablenot availableconditions_1
HCCH-COSYnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
15N-NOESY-HSQCnot availablenot availableconditions_1
13C-NOESY-HSQCnot availablenot availableconditions_1
HNHAnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance DMX 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAB26281 BAE39778 BAG63244 BAN82198
EMBL CAA06462
GB AAH17309 AAH28860 AAI46083 AHW40493 EAW86374
PRF 1713233A
REF NP_001094569 NP_001102999 NP_001248742 NP_001278594 NP_036443
SP O60870 Q8K339
TPG DAA23636
AlphaFold O60870 Q8K339

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks