BMRB Entry 6936
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6936
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Citation: Harris, Richard; Bayliss, Richard; Waksman, Gabriel; Driscoll, Paul. "Resonance Assignments of the Complex between TraN and the C-terminal Domain of
TraO from the Conjugative Plasmid pKM101" J. Biomol. NMR 36, 31-31 (2006).
PubMed: 16601857
Assembly members:
VirB7, polymer, 36 residues, 3772 Da.
VirB9, polymer, 126 residues, 14060 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 634 |
| 15N chemical shifts | 151 |
| 1H chemical shifts | 1016 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VirB7 | 1 |
| 2 | VirB9 | 2 |
Entities:
Entity 1, VirB7 36 residues - 3772 Da.
GAMA is part of the tag and no chemical shifts for the tag residues are reported. First VirB7 amino acid is S5 which in the proper sequence is residue number 17 as given in the chemical shift list.
| 1 | GLY | ALA | MET | ALA | SER | GLY | HIS | LYS | PRO | PRO | ||||
| 2 | PRO | GLU | PRO | ASP | TRP | SER | ASN | THR | VAL | PRO | ||||
| 3 | VAL | ASN | LYS | THR | ILE | PRO | VAL | ASP | THR | GLN | ||||
| 4 | GLY | GLY | ARG | ASN | GLU | SER |
Entity 2, VirB9 126 residues - 14060 Da.
GIDPFTHM is part of the tag and no chemical shifts for the tag residues are reported. First VirB9 amino acid is A9 which in the proper sequence is residue number 177 as given in the chemical shift list.
| 1 | GLY | ILE | ASP | PRO | PHE | THR | HIS | MET | ALA | GLY | ||||
| 2 | ALA | LYS | ASN | TYR | GLN | TYR | VAL | MET | SER | GLU | ||||
| 3 | GLN | PRO | GLU | MET | ARG | SER | ILE | GLN | PRO | VAL | ||||
| 4 | HIS | VAL | TRP | ASP | ASN | TYR | ARG | PHE | THR | ARG | ||||
| 5 | PHE | GLU | PHE | PRO | ALA | ASN | ALA | GLU | LEU | PRO | ||||
| 6 | GLN | VAL | TYR | MET | ILE | SER | ALA | SER | GLY | LYS | ||||
| 7 | GLU | THR | LEU | PRO | ASN | SER | HIS | VAL | VAL | GLY | ||||
| 8 | GLU | ASN | ARG | ASN | ILE | ILE | GLU | VAL | GLU | THR | ||||
| 9 | VAL | ALA | LYS | GLU | TRP | ARG | ILE | ARG | LEU | GLY | ||||
| 10 | ASP | LYS | VAL | VAL | GLY | VAL | ARG | ASN | ASN | ASN | ||||
| 11 | PHE | ALA | PRO | GLY | ALA | GLY | ALA | VAL | ALA | THR | ||||
| 12 | GLY | THR | ALA | SER | PRO | ASP | VAL | ARG | ARG | VAL | ||||
| 13 | GLN | ILE | GLY | GLU | ASP | ASN |
Samples:
sample_1: VirB7, [U-15N], 1 mM; VirB9 1 mM
sample_2: VirB7, [U-13C; U-15N], 1 mM; VirB9 1 mM
sample_3: VirB7 1 mM; VirB9, [U-15N], 1 mM
sample_4: VirB7 1 mM; VirB9, [U-13C; U-15N], 1 mM
conditions_1: pH: 6.7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H 15N HSQC | not available | not available | conditions_1 |
| 1H 13C HSQC | not available | not available | conditions_1 |
| 1H 13C CT-HSQC | not available | not available | conditions_1 |
| 1H 15N TOCSY-HSQC | not available | not available | conditions_1 |
| 1H 15N NOESY-HSQC | not available | not available | conditions_1 |
| HNCO | not available | not available | conditions_1 |
| HN(CA)CO | not available | not available | conditions_1 |
| HNCA | not available | not available | conditions_1 |
| HN(CO)CA | not available | not available | conditions_1 |
| HNCACB | not available | not available | conditions_1 |
| CBCA(CO)NH | not available | not available | conditions_1 |
| HA(CA)NH | not available | not available | conditions_1 |
| HA(CACO)NH | not available | not available | conditions_1 |
| HCCH-TOCSY | not available | not available | conditions_1 |
| HCCH-COSY | not available | not available | conditions_1 |
| 1H 13C NOESY-HSQC | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity Plus 500 MHz
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAF38206 BAF93109 BAM29017 |
| EMBL | CCA62524 CCE73953 CCN79904 CDF32038 CDK67343 |
| GB | AAA86458 AAL13392 ABG33818 ABG49204 ABO42055 |
| REF | NP_511196 WP_000735066 WP_000735067 WP_010999963 WP_013279390 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks