BMRB Entry 6928

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6928

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Title:
1H, 13C, and 15N NMR Assignment of the Rep Protein Nuclease Domain from the Porcine Circovirus PCV2
Deposition date:
2005-12-21
Original release date:
2006-05-02
Authors:
Vega, Susana; Campos-Olivas, Ramon
Citation:

Citation: Rocha, Susana; Campos-Olivas, Ramon. "1H, 13C, and 15N NMR Assignment of the Rep Protein Nuclease Domain from the Porcine Circovirus PCV2"  J. Biomol. NMR 36, 25-25 (2006).
PubMed: 16636755

Assembly members:

Assembly members:
N-terminal DNA binding domain of Rep protein from the circovirus PCV2, polymer, 115 residues, 13251.9 Da.

Natural source:

Natural source:   Common Name: Porcine circovirus 2   Taxonomy ID: 46221   Superkingdom: Viruses   Kingdom: not available   Genus/species: Circovirus Porcine circovirus 2

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal DNA binding domain of Rep protein from the circovirus PCV2: PSKKNGRSGPQPHKRWVFTL NNPSEDERKKIRDLPISLFD YFIVGEEGNEEGRTPHLQGF ANFVKKQTFNKVKWYLGARC HIEKAKGTDQQNKEYCSKEG NLLMECGAPRSQGQR

Data sets:
Data typeCount
13C chemical shifts498
15N chemical shifts125
1H chemical shifts752

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PCV21

Entities:

Entity 1, PCV2 115 residues - 13251.9 Da.

1   PROSERLYSLYSASNGLYARGSERGLYPRO
2   GLNPROHISLYSARGTRPVALPHETHRLEU
3   ASNASNPROSERGLUASPGLUARGLYSLYS
4   ILEARGASPLEUPROILESERLEUPHEASP
5   TYRPHEILEVALGLYGLUGLUGLYASNGLU
6   GLUGLYARGTHRPROHISLEUGLNGLYPHE
7   ALAASNPHEVALLYSLYSGLNTHRPHEASN
8   LYSVALLYSTRPTYRLEUGLYALAARGCYS
9   HISILEGLULYSALALYSGLYTHRASPGLN
10   GLNASNLYSGLUTYRCYSSERLYSGLUGLY
11   ASNLEULEUMETGLUCYSGLYALAPROARG
12   SERGLNGLYGLNARG

Samples:

sample_1: Rep PCV2 2-116, [U-10% 13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 8%

sample_2: Rep PCV2 2-116, [U-10% 13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 100%

sample_3: Rep PCV2 2-116, [U-13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 8%

sample_4: Rep PCV2 2-116, [U-13C; U-15N], 0.8 M; Sodium phosphate 20 mM; NaCl 100 mM; DTT 1 mM; D2O 100%

conditions_1: ionic strength: 0.264 M; pH: 6.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D_1H15N_HSQCnot availablenot availableconditions_1
2D_1H15N_HSQC_long_rangenot availablenot availableconditions_1
2D_1H13C_HSQC/HMQCnot availablenot availableconditions_1
2D_HNCOCG_aromnot availablenot availableconditions_1
2D_HNCO_aromnot availablenot availableconditions_1
3D_HNCOnot availablenot availableconditions_1
3D_HNCACBnot availablenot availableconditions_1
3D_CBCA_(CO)_NHnot availablenot availableconditions_1
3D_HNHAnot availablenot availableconditions_1
3D_HNHBnot availablenot availableconditions_1
3D_15N_edited_NOESYnot availablenot availableconditions_1
4D_15N,_13C_NOESYnot availablenot availableconditions_1
4D_13C,_13C_NOESYnot availablenot availableconditions_1
3D_HCACOnot availablenot availableconditions_1
3D_CC_(CO)_NHnot availablenot availableconditions_1
HCCH_TOCSYnot availablenot availableconditions_1
3D_15N_edited_TOCSYnot availablenot availableconditions_1

Software:

NMRView v5.0.20 -

NMRPipe v97.027.12.56 -

NMR spectrometers:

  • Bruker AMX Avance 600 MHz
  • Bruker AMX Avance 700 MHz

Related Database Links:

PDB
DBJ BAA88133 BAB69432 BAB69437 BAB69441 BAG12761
EMBL CAA11157 CAC41083 CAC41084 CAC41085 CAF25171
GB AAC35298 AAC35309 AAC35320 AAC35330 AAC59462
REF NP_937956
SP Q8BB16
AlphaFold Q8BB16

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks