BMRB Entry 6914

Name: Solution structure of the Wilson ATPase N-domain in the presence of ATP
Published: 2007-02-09

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PDB ID: 2arf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6914
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Wilson ATPase N-domain in the presence of ATP

Deposition date:

2005-12-01

Original release date:

2007-02-09

Authors:

Dmitriev, O.; Tsivkovski, R.; Abildgaard, F.; Morgan, C.; Markley, J.; Lutsenko, S.

Citation:

Citation: Dmitriev, O.; Tsivkovski, R.; Abildgaard, F.; Lutsenko, S.. "NMR assignment of the Wilson disease associated protein N-domain" J. Biomol. NMR 36, 61-61 (2006).
PubMed: 16868859

Assembly members:

Assembly members:
Wilson Disease ATPase N-domain, polymer, 165 residues, 17292 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Wilson Disease ATPase N-domain: AGHMVPRVMRVLLLGDVATL PLRKVLAVVGTAEASSEHPL GVAVTKYCKEELGTETLGYC TDFQAVPGCGIGCKVSNVEG ILAHSERPLSAPASHLNEAG SLPAEKDAVPQTFSVLIGNR EWLRRNGLTISSDVSDAMTD HEMKGQTAILVAIDGVLCGM IAIAD

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	645
15N chemical shifts	164
1H chemical shifts	1019

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WILSON DISEASE ATPASE, N-domain	1

Entities:

Entity 1, WILSON DISEASE ATPASE, N-domain 165 residues - 17292 Da.

1

ALA

GLY

HIS

MET

VAL

PRO

ARG

VAL

MET

ARG

2

VAL

LEU

GLY

ASP

VAL

ALA

THR

LEU

3

PRO

LEU

ARG

LYS

VAL

LEU

ALA

VAL

GLY

4

THR

ALA

GLU

ALA

SER

GLU

HIS

PRO

LEU

5

GLY

VAL

ALA

VAL

THR

LYS

TYR

CYS

LYS

GLU

6

GLU

LEU

GLY

THR

GLU

THR

LEU

GLY

TYR

CYS

7

THR

ASP

PHE

GLN

ALA

VAL

PRO

GLY

CYS

GLY

8

ILE

GLY

CYS

LYS

VAL

SER

ASN

VAL

GLU

GLY

9

ILE

LEU

ALA

HIS

SER

GLU

ARG

PRO

LEU

SER

10

ALA

PRO

ALA

SER

HIS

LEU

ASN

GLU

ALA

GLY

11

SER

LEU

PRO

ALA

GLU

LYS

ASP

ALA

VAL

PRO

12

GLN

THR

PHE

SER

VAL

LEU

ILE

GLY

ASN

ARG

13

GLU

TRP

LEU

ARG

ASN

GLY

LEU

THR

ILE

14

SER

ASP

VAL

SER

ASP

ALA

MET

THR

ASP

15

HIS

GLU

MET

LYS

GLY

GLN

THR

ALA

ILE

LEU

16

VAL

ALA

ILE

ASP

GLY

VAL

LEU

CYS

GLY

MET

17

ILE

ALA

ILE

ALA

ASP

Samples:

sample_1: Wilson Disease ATPase N-domain, [U-99% 13C; U-95% 15N], 0.75 mM; sodium phosphate 50 mM; DTT 5 mM; NaN3 50 mM; ATP 5 mM; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	sample_cond_1
3D 13C-separated NOESY	sample_1	not available	sample_cond_1
4D 13C/15N-separated NOESY	sample_1	not available	sample_cond_1

Software:

xwinnmr v3.5 - collection

FELIX v2000 - data analysis

CYANA v1.0 - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 600 MHz

BMRB	16761
PDB	2ARF
DBJ	BAD92698 BAG63463
GB	AAA79212 AAA92667 AAB52902 AAI17201 AAI43974
PIR	S40525
PRF	2001422A
REF	NP_000044 NP_001005918 NP_001230111 XP_001103242 XP_003270167
SP	P35670
AlphaFold	P35670