BMRB Entry 6913
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6913
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Citation: Pinheiro, Anderson; Amorim, Gisele; Netto, Luis Eduardo; Valente, Ana Paula; Almeida, Fabio. "1H, 13C and 15N Resonance Assignments for the Reduced Forms of Thioredoxin 1
and 2 from S. cerevisiae" J. Biomol. NMR 36, 35-35 (2006).
PubMed: 16609834
Assembly members:
thioredoxin 2, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
thioredoxin 2: MVTQLKSASEYDSALASGDK
LVVVDFFATWCGPCKMIAPM
IEKFAEQYSDAAFYKLDVDE
VSDVAQKAEVSSMPTLIFYK
GGKEVTRVVGANPAAIKQAI
ASNV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 435 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 700 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | thioredoxin | 1 |
Entities:
Entity 1, thioredoxin 104 residues - Formula weight is not available
| 1 | MET | VAL | THR | GLN | LEU | LYS | SER | ALA | SER | GLU | ||||
| 2 | TYR | ASP | SER | ALA | LEU | ALA | SER | GLY | ASP | LYS | ||||
| 3 | LEU | VAL | VAL | VAL | ASP | PHE | PHE | ALA | THR | TRP | ||||
| 4 | CYS | GLY | PRO | CYS | LYS | MET | ILE | ALA | PRO | MET | ||||
| 5 | ILE | GLU | LYS | PHE | ALA | GLU | GLN | TYR | SER | ASP | ||||
| 6 | ALA | ALA | PHE | TYR | LYS | LEU | ASP | VAL | ASP | GLU | ||||
| 7 | VAL | SER | ASP | VAL | ALA | GLN | LYS | ALA | GLU | VAL | ||||
| 8 | SER | SER | MET | PRO | THR | LEU | ILE | PHE | TYR | LYS | ||||
| 9 | GLY | GLY | LYS | GLU | VAL | THR | ARG | VAL | VAL | GLY | ||||
| 10 | ALA | ASN | PRO | ALA | ALA | ILE | LYS | GLN | ALA | ILE | ||||
| 11 | ALA | SER | ASN | VAL |
Samples:
sample_1: thioredoxin 2, [U-13C; U-15N], 1.2 mM; 2-mercaptoethanol 10 mM; H2O 90%; D2O 10%
sample_2: thioredoxin 2, [U-15N], 1.2 mM; DTT 10 mM; H2O 90%; D2O 10%
sample_3: thioredoxin 2 1.2 mM; DTT 10 mM; D2O 99%
conditions_1: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | conditions_1 |
| HNCA | not available | not available | conditions_1 |
| HNCOCA | not available | not available | conditions_1 |
| HNCACB | not available | not available | conditions_1 |
| CBCACONH | not available | not available | conditions_1 |
| HCCH-TOCSY | not available | not available | conditions_1 |
| HCCCONH | not available | not available | conditions_1 |
| CCCONH | not available | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA23581 |
| EMBL | CAA89002 CAA97236 CAY79968 |
| GB | AAA35170 AAA35178 AAA85584 AAS56143 AHY79550 |
| REF | NP_011725 |
| SP | P22803 |
| TPG | DAA08303 |
| AlphaFold | P22803 |
Download HSQC peak lists in one of the following formats:
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