BMRB Entry 6911
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6911
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Charbonnier, Sebastian; Coutouly, Marie-Aude; Kieffer, Bruno; Trave, Gilles; Atkinson, R.. "13C, 15N and 1H Resonance Assignment of the PDZ1 domain of MAGI-1 using QUASI" J. Biomol. NMR 36, 33-33 (2006).
PubMed: 16636753
Assembly members:
MAGI PDZ1, polymer, 129 residues, 13855.8 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Vector: PETM-41
Entity Sequences (FASTA):
MAGI PDZ1: GAMGKPFFTRNPSELKGKFI
HTKLRKSSRGFGFTVVGGDE
PDEFLQIKSLVLDGPAALDG
KMETGDVIVSVNDTCVLGHT
HAQVVKIFQSIPIGASVDLE
LCRGYPLPFDPDDPNTSLVT
SVAILDKEP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 369 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 765 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MAGI-1 PDZ1 | 1 |
Entities:
Entity 1, MAGI-1 PDZ1 129 residues - 13855.8 Da.
| 1 | GLY | ALA | MET | GLY | LYS | PRO | PHE | PHE | THR | ARG | ||||
| 2 | ASN | PRO | SER | GLU | LEU | LYS | GLY | LYS | PHE | ILE | ||||
| 3 | HIS | THR | LYS | LEU | ARG | LYS | SER | SER | ARG | GLY | ||||
| 4 | PHE | GLY | PHE | THR | VAL | VAL | GLY | GLY | ASP | GLU | ||||
| 5 | PRO | ASP | GLU | PHE | LEU | GLN | ILE | LYS | SER | LEU | ||||
| 6 | VAL | LEU | ASP | GLY | PRO | ALA | ALA | LEU | ASP | GLY | ||||
| 7 | LYS | MET | GLU | THR | GLY | ASP | VAL | ILE | VAL | SER | ||||
| 8 | VAL | ASN | ASP | THR | CYS | VAL | LEU | GLY | HIS | THR | ||||
| 9 | HIS | ALA | GLN | VAL | VAL | LYS | ILE | PHE | GLN | SER | ||||
| 10 | ILE | PRO | ILE | GLY | ALA | SER | VAL | ASP | LEU | GLU | ||||
| 11 | LEU | CYS | ARG | GLY | TYR | PRO | LEU | PRO | PHE | ASP | ||||
| 12 | PRO | ASP | ASP | PRO | ASN | THR | SER | LEU | VAL | THR | ||||
| 13 | SER | VAL | ALA | ILE | LEU | ASP | LYS | GLU | PRO |
Samples:
sample_1: MAGI-1 PDZ1, [U-100% 13C; U-100% 15N], 0.125 ± 0.01 mM; Tris-HCl 25 ± 0.1 mM; NaCl 50 ± 0.1 mM
conditions_1: pH: 6.8; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| 15N-edited_NOESY | sample_1 | not available | conditions_1 |
| 15N-edited_TOCSY | sample_1 | not available | conditions_1 |
| 1H13C_HSQC | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HN(CO)CACB | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HN(CA)CO | sample_1 | not available | conditions_1 |
| HCCH-COSY | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| 13C-edited_NOESY | sample_1 | not available | conditions_1 |
Software:
xwinnmr v2.6, Bruker - acquisition
NMRPipe, NIH - processing
CARA, ETH - analysis
QUASI, UMR7104 -
NMR spectrometers:
- Bruker DRX Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks