BMRB Entry 6900

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6900

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Title:
15N, 13C and 1H resonance assignments for the C-terminal MA-3 domain of the tumour suppressor protein Pdcd4
Deposition date:
2005-11-14
Original release date:
2006-03-10
Authors:
Waters, Lorna; Bohm, Maret; Veverka, Vaclav; Muskett, Fred; Frenkiel, Thomas; Kelly, Geoff; Prescott, Andrew; Dosanjh, Nuvjeevan; Klempnauer, Karl-Heinz; Carr, Mark
Citation:

Citation: Waters, Lorna; Bohm, Maret; Veverka, Vaclav; Muskett, Frederick; Frenkiel, Thomas; Kelly, Geoffrey; Prescott, Andrew; Dosanjh, Nuvjeevan; Klempnauer, Karl-Heinz; Carr, Mark. "NMR Assignment and Secondary Structure Determination of the C-terminal MA-3 Domain of the Tumour Suppressor Protein Pdcd4"  J. Biomol. NMR 36, 18-18 (2006).
PubMed: 16518566

Assembly members:

Assembly members:
Pdcd4 MA-3, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pdcd4 MA-3: GPLGSGGQQPVNHLVKEIDM LLKEYLLSGDISEAEHCLKE LEVPHFHHELVYEAIVMVLE STGESAFKMILDLLKSLWKS STITIDQMKRGYERIYNEIP DINLDVPHSYSVLERFVEEC FQAGIISKQLRDLCPS

Data sets:
Data typeCount
13C chemical shifts435
15N chemical shifts130
1H chemical shifts985

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pdcd4 C-terminal MA-3 domain1

Entities:

Entity 1, Pdcd4 C-terminal MA-3 domain 136 residues - Formula weight is not available

1   GLYPROLEUGLYSERGLYGLYGLNGLNPRO
2   VALASNHISLEUVALLYSGLUILEASPMET
3   LEULEULYSGLUTYRLEULEUSERGLYASP
4   ILESERGLUALAGLUHISCYSLEULYSGLU
5   LEUGLUVALPROHISPHEHISHISGLULEU
6   VALTYRGLUALAILEVALMETVALLEUGLU
7   SERTHRGLYGLUSERALAPHELYSMETILE
8   LEUASPLEULEULYSSERLEUTRPLYSSER
9   SERTHRILETHRILEASPGLNMETLYSARG
10   GLYTYRGLUARGILETYRASNGLUILEPRO
11   ASPILEASNLEUASPVALPROHISSERTYR
12   SERVALLEUGLUARGPHEVALGLUGLUCYS
13   PHEGLNALAGLYILEILESERLYSGLNLEU
14   ARGASPLEUCYSPROSER

Samples:

sample_1: Pdcd4 MA-3, [U-15N], 0.6 mM; sodium chloride 100 mM; sodium phosphate 25 mM; DTT 0.5 mM; EDTA 10 uM

sample_2: Pdcd4 MA-3, [U-15N; U-13C], 0.7 mM; sodium chloride 100 mM; sodium phosphate 25 mM; DTT 0.5 mM; EDTA 10 uM

sample_3: Pdcd4 MA-3, [U-13C], 0.9 mM; sodium chloride 100 mM; sodium phosphate 25 mM; DTT 0.5 mM; EDTA 10 uM

sample_4: Pdcd4 MA-3 1 mM; sodium chloride 100 mM; sodium phosphate 25 mM; DTT 0.5 mM; EDTA 10 uM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H13C_HSQCsample_3not availableconditions_1
1H15N13C_HNCACBsample_2not availableconditions_1
1H15N13C_CBCA(CO)NHsample_2not availableconditions_1
1H15N_NOESYHSQCsample_1not availableconditions_1
1H15N_TOCSYHSQCsample_1not availableconditions_1
1H13C_HCCHTOCSYsample_2not availableconditions_1
1H13C_NOESYHSQCsample_3not availableconditions_1
1H_NOESYsample_4not availableconditions_1
1H_TOCSYsample_4not availableconditions_1

Software:

TOPSPIN v1.2, Bruker BioSpin Ltd - Acquistion and Processing of NMR data

SPARKY v3.110, T. D. Goddard and D. G. Kneller - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DRX 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAK63524
REF XP_005269759 XP_006717830 XP_008949157

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks