BMRB Entry 6895

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6895
MolProbity Validation Chart

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Title:
NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU
Deposition date:
2005-11-10
Original release date:
2006-02-23
Authors:
Allain, F.; Auweter, S.
Citation:

Citation: Auweter, S.; Fasan, R.; Reymond, L.; Underwood, J.; Black, D.; Pitsch, S.; Allain, F.. "Molecular basis of RNA recognition by the human alternative splicing factor Fox-1."  EMBO J. 25, 163-173 (2006).
PubMed: 16362037

Assembly members:

Assembly members:
Ataxin-2-binding protein 1, polymer, 109 residues, Formula weight is not available
UGCAUGU, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ataxin-2-binding protein 1: MGSSHHHHHHSSGLVPRGSH MNTENKSQPKRLHVSNIPFR FRDPDLRQMFGQFGKILDVE IIFNERGSKGFGFVTFENSA DADRAREKLHGTVVEGRKIE VNNATARVM
UGCAUGU: UGCAUGU

Data sets:
Data typeCount
13C chemical shifts234
15N chemical shifts84
1H chemical shifts564

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ataxin-2-binding protein 11
2UGCAUGU2

Entities:

Entity 1, Ataxin-2-binding protein 1 109 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METASNTHRGLUASNLYSSERGLNPROLYS
4   ARGLEUHISVALSERASNILEPROPHEARG
5   PHEARGASPPROASPLEUARGGLNMETPHE
6   GLYGLNPHEGLYLYSILELEUASPVALGLU
7   ILEILEPHEASNGLUARGGLYSERLYSGLY
8   PHEGLYPHEVALTHRPHEGLUASNSERALA
9   ASPALAASPARGALAARGGLULYSLEUHIS
10   GLYTHRVALVALGLUGLYARGLYSILEGLU
11   VALASNASNALATHRALAARGVALMET

Entity 2, UGCAUGU 7 residues - Formula weight is not available

1   UGCAUGU

Samples:

sample_1: Ataxin-2-binding protein 1, [U-15N], 1 mM; UGCAUGU 1 mM; sodium phoshphate 20 mM; NaCl 30 mM; H2O 90%; D2O 10%

sample_2: Ataxin-2-binding protein 1, [U-13C; U-15N], 1 mM; UGCAUGU 1 mM; sodium phoshphate 20 mM; NaCl 30 mM; H2O 90%; D2O 10%

sample_3: Ataxin-2-binding protein 1, [U-15N], 1 mM; UGCAUGU 1 mM; sodium phoshphate 20 mM; NaCl 30 mM; D2O 100%

sample_4: Ataxin-2-binding protein 1, [U-13C; U-15N], 1 mM; UGCAUGU 1 mM; sodium phoshphate 20 mM; NaCl 30 mM; D2O 100%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
2D F1not availablenot availablenot available
F2-edited NOESYnot availablenot availablenot available
2D F1-editednot availablenot availablenot available
F2-fitered NOESYnot availablenot availablenot available

Software:

CYANA v2.0 - structure solution

AMBER v7.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAA91472 BAA95079 BAB46877 BAD92063 BAE22304
EMBL CAD28499 CAH89770 CAH91712 CAI29625
GB AAF78291 AAH59002 AAI13692 AAI43817 AAL71904
REF NP_001100444 NP_001125995 NP_001135805 NP_001135806 NP_061193
SP Q5NVN8 Q95KI0 Q9JJ43 Q9NWB1
AlphaFold Q5NVN8 Q95KI0 Q9JJ43 Q9NWB1

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SPARKY: Backbone or all simulated peaks