BMRB Entry 6879

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignment for aKv1.1N
Published: 2006-02-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6879

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignment for aKv1.1N

Deposition date:

2005-10-27

Original release date:

2006-02-17

Authors:

Baker, Kent; Hilty, Christian; Peti, Wolfgang; Pfaffinger, Paul; Wider, Gerhard; Wuthrich, Kurt; Choe, Senyon

Citation:

Citation: Baker, Kent; Hilty, Christian; Peti, Wolfgang; Pfaffinger, Paul; Wider, Gerhard; Wuthrich, Kurt; Choe, Senyon. "NMR-Derived Dynamic Aspects of N-Type Inactivation of a Kv Channel Suggest a Transient Interaction with the T1 Domain" Biochemistry 45, 1663-1672 (2006).
PubMed: 16460013

Assembly members:

Assembly members:
aKv1.1, polymer, 181 residues, 21012 Da.

Natural source:

Natural source: Common Name: California sea hare Taxonomy ID: 6500 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Aplysia Aplysia californica

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
aKv1.1: MEVAMAGIEGNGGPAGYRDS YHSSQRPLLRSSNLPNSRSF PKLSEEDNANENGMGVPGSD YDCSCERVVINVSGLRFETQ LKTLNQFPDTLLGNPQKRNR YYDPLRNEYFFDRNRPSFDA ILYFYQSGGRLRRPVNVPLD VFSEEIKFYELGENAFERYR EDEGFIKEEEKPLPQNEFQR R

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	142
15N chemical shifts	142
1H chemical shifts	142

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	aKv1.1N, chain 1	1
2	aKv1.1N, chain 2	1
3	aKv1.1N, chain 3	1
4	aKv1.1N, chain 4	1

Entities:

Entity 1, aKv1.1N, chain 1 181 residues - 21012 Da.

1

MET

GLU

VAL

ALA

MET

ALA

GLY

ILE

GLU

GLY

2

ASN

GLY

PRO

ALA

GLY

TYR

ARG

ASP

SER

3

TYR

HIS

SER

GLN

ARG

PRO

LEU

ARG

4

SER

ASN

LEU

PRO

ASN

SER

ARG

SER

PHE

5

PRO

LYS

LEU

SER

GLU

ASP

ASN

ALA

ASN

6

GLU

ASN

GLY

MET

GLY

VAL

PRO

GLY

SER

ASP

7

TYR

ASP

CYS

SER

CYS

GLU

ARG

VAL

ILE

8

ASN

VAL

SER

GLY

LEU

ARG

PHE

GLU

THR

GLN

9

LEU

LYS

THR

LEU

ASN

GLN

PHE

PRO

ASP

THR

10

LEU

GLY

ASN

PRO

GLN

LYS

ARG

ASN

ARG

11

TYR

ASP

PRO

LEU

ARG

ASN

GLU

TYR

PHE

12

PHE

ASP

ARG

ASN

ARG

PRO

SER

PHE

ASP

ALA

13

ILE

LEU

TYR

PHE

TYR

GLN

SER

GLY

ARG

14

LEU

ARG

PRO

VAL

ASN

VAL

PRO

LEU

ASP

15

VAL

PHE

SER

GLU

ILE

LYS

PHE

TYR

GLU

16

LEU

GLY

GLU

ASN

ALA

PHE

GLU

ARG

TYR

ARG

17

GLU

ASP

GLU

GLY

PHE

ILE

LYS

GLU

18

LYS

PRO

LEU

PRO

GLN

ASN

GLU

PHE

GLN

ARG

19

ARG

Samples:

sample_1: aKv1.1, [U-2H; U-13C; U-15N], 1.0 mM; Sodium Phosphate 20 mM; NaCl 50 mM

conditions_1: pH: 7.0; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
(15N_1H)TROSY	sample_1	not available	conditions_1
(1H_1H)NOESY-(15N_1N)-TROSY	sample_1	not available	conditions_1
TROSY-HNCA	sample_1	not available	conditions_1
(H)N(COCA)NH	sample_1	not available	conditions_1

Software:

xwinnmr, Bruker - Collection

XEASY - Processing

CARA - Processing

NMR spectrometers:

Bruker . 900 MHz
Bruker . 800 MHz
Bruker . 500 MHz

PDB	1A68 1EOD 1EOE 1EOF 1T1D
GB	AAA27756
REF	NP_001191634