BMRB Entry 6869

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6869
MolProbity Validation Chart

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Title:
Solution Structure of C-Terminal 14 kDa Domain of the tau subunit from Escherichia coli DNA Polymerase III
Deposition date:
2005-10-20
Original release date:
2007-11-15
Authors:
Jergic, S.; Dixon, N.; Otting, G.; Su, X.
Citation:

Citation: Jergic, S.; Ozawa, K.; Williams, N.; Su, X.; Scott, D.; Hamdan, S.; Crowther, J.; Otting, G.; Dixon, N.. "The unstructured C-terminus of the {tau} subunit of Escherichia coli DNA polymerase III holoenzyme is the site of interaction with the {alpha} subunit"  Nucleic Acids Res. 35, 2813-2824 (2007).
PubMed: 17355988

Assembly members:

Assembly members:
DNA polymerase III subunit tau, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA polymerase III subunit tau: MKALEHEKTPELAAKLAAEA IERDPWAAQVSQLSLPKLVE QVALNAWKEESDNAVCLHLR SSQRHLNNRGAQQKLAEALS MLKGSTVELTIVEDDNPAVR TPLEWRQAIYEEKLAQARES IIADNNIQ

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts135
1H chemical shifts898

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA polymerase III subunit tau1

Entities:

Entity 1, DNA polymerase III subunit tau 128 residues - Formula weight is not available

1   METLYSALALEUGLUHISGLULYSTHRPRO
2   GLULEUALAALALYSLEUALAALAGLUALA
3   ILEGLUARGASPPROTRPALAALAGLNVAL
4   SERGLNLEUSERLEUPROLYSLEUVALGLU
5   GLNVALALALEUASNALATRPLYSGLUGLU
6   SERASPASNALAVALCYSLEUHISLEUARG
7   SERSERGLNARGHISLEUASNASNARGGLY
8   ALAGLNGLNLYSLEUALAGLUALALEUSER
9   METLEULYSGLYSERTHRVALGLULEUTHR
10   ILEVALGLUASPASPASNPROALAVALARG
11   THRPROLEUGLUTRPARGGLNALAILETYR
12   GLUGLULYSLEUALAGLNALAARGGLUSER
13   ILEILEALAASPASNASNILEGLN

Samples:

sample_1: DNA polymerase III subunit tau, [U-15N; U-13C], 1.8 mM; phosphate buffer 5 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_2: DNA polymerase III subunit tau, [U-10% 13C], 0.6 mM; phosphate buffer 5 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_3: DNA polymerase III subunit tau 1.8 mM; phosphate buffer 5 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_4: DNA polymerase III subunit tau 1.8 mM; phosphate buffer 5 mM; NaCl 100 mM; D2O 100%

sample_cond_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CACB(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

TOPSPIN v1.3 - processing

DYANA v1.5 - structure solution

PROSA v6.1 - processing

OPAL v2.6 - refinement

CARA v1.2 - data analysis

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB33946 BAE76249 BAG76019 BAI23844 BAI29314
EMBL CAA27827 CAA28174 CAA28175 CAP75003 CAQ30943
GB AAA23457 AAB40224 AAC73572 AAG54819 AAN42070
REF NP_286211 NP_308550 NP_415003 NP_706363 NP_752523
SP P06710
AlphaFold P06710

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks