BMRB Entry 6868

Title:
Solution Structure of ydhR protein from Escherichia coli   PubMed: 16260765
Deposition date:
2005-10-20
Original release date:
2006-04-17
Authors:
Revington, M.; Shaw, G.
Citation:

Citation: Revington, M.; Semesi, A.; Yee, A.; Shaw, G.. "Solution structure of the Escherichia coli protein ydhR: a putative mono-oxygenase"  Protein Sci. 14, 3115-3120 (2005).

Assembly members:

Assembly members:
Protein ydhR precursor, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts108
1H chemical shifts690

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein ydhR precursor, chain A1
2Protein ydhR precursor, chain B1

Entities:

Entity 1, Protein ydhR precursor, chain A 123 residues - Formula weight is not available

1   METGLYTHRSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETALATHRLEULEUGLNLEUHIS
4   PHEALAPHEASNGLYPROPHEGLYASPALA
5   METALAGLUGLNLEULYSPROLEUALAGLU
6   SERILEASNGLNGLUPROGLYPHELEUTRP
7   LYSVALTRPTHRGLUSERGLULYSASNHIS
8   GLUALAGLYGLYILETYRLEUPHETHRASP
9   GLULYSSERALALEUALATYRLEUGLULYS
10   HISTHRALAARGLEULYSASNLEUGLYVAL
11   GLUGLUVALVALALALYSVALPHEASPVAL
12   ASNGLUPROLEUSERGLNILEASNGLNALA
13   LYSLEUALA

Samples:

sample_1: Protein ydhR precursor, [U-15N; U-13C], 0.5 mM; MES 25 mM; NaCl 450 mM; Sodium Azide 0.01%; H2O 90%; D2O 10%

sample_2: Protein ydhR precursor, [U-15N; U-13C], 0.5 mM; MES 25 mM; NaCl 450 mM; Sodium Azide 0.01%; D2O 100%

sample_cond_1: ionic strength: 450 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-aliphatic region-separated NOESYnot availablenot availablenot available
3D 13C-aromatic region-separated NOESYnot availablenot availablenot available

Software:

VNMR v6.1B - collection

NMRPipe v2.3 - processing

NMRView v5.2.2 - data analysis

CYANA v2.032.3 - refinement

CNS v1.1 - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB35797 BAE76495 BAG77313 BAI25642 BAI30616
EMBL CAP76164 CAQ32143 CAQ98575 CAR03026 CAR13152
GB AAB47943 AAC74737 AAG56654 AAN43273 AAP17161
REF NP_288101 NP_310401 NP_416182 NP_707566 NP_837352
SP P0ACX3 P0ACX4
AlphaFold P0ACX4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks