BMRB Entry 6864

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6864

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments of a repeat domain of the egg case silk from Nephila antipodiana

Deposition date:

2005-10-16

Original release date:

2006-04-24

Authors:

Lin, Zhi; Huang, Weidong; Yang, Daiwen

Citation:

Citation: Lin, Zhi; Huang, Weidong; Yang, Daiwen. "Resonance Assignments of a Repeated Domain of the Egg Case Silk from Nephila Antipodiana" J. Biomol. NMR 36, 17-17 (2006).
PubMed: 16601859

Assembly members:

Assembly members:
tu81d5, polymer, 162 residues, Formula weight is not available

Natural source:

Natural source: Common Name: golden web spider Taxonomy ID: 6893 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Arachnida araneae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tu81d5: GSSGATSQAASQSASSSYSS AFAQAASSALATSSAISRAF ASVSSASAASSLAYNIGLSA ARSLGIASDTALAGALAQAV GGVGAGASASAYANAIARAA GQFLATQGVLNAGNASALAG SFARALSASAESQSFAQSQA YQQASAFQQAAAQSAAQSAS RA

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	408
15N chemical shifts	169
1H chemical shifts	893

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tu81d5	1

Entities:

Entity 1, tu81d5 162 residues - Formula weight is not available

1

GLY

SER

GLY

ALA

THR

SER

GLN

ALA

2

SER

GLN

SER

ALA

SER

TYR

SER

3

ALA

PHE

ALA

GLN

ALA

SER

ALA

LEU

4

ALA

THR

SER

ALA

ILE

SER

ARG

ALA

PHE

5

ALA

SER

VAL

SER

ALA

SER

ALA

SER

6

SER

LEU

ALA

TYR

ASN

ILE

GLY

LEU

SER

ALA

7

ALA

ARG

SER

LEU

GLY

ILE

ALA

SER

ASP

THR

8

ALA

LEU

ALA

GLY

ALA

LEU

ALA

GLN

ALA

VAL

9

GLY

VAL

GLY

ALA

GLY

ALA

SER

ALA

SER

10

ALA

TYR

ALA

ASN

ALA

ILE

ALA

ARG

ALA

11

GLY

GLN

PHE

LEU

ALA

THR

GLN

GLY

VAL

LEU

12

ASN

ALA

GLY

ASN

ALA

SER

ALA

LEU

ALA

GLY

13

SER

PHE

ALA

ARG

ALA

LEU

SER

ALA

SER

ALA

14

GLU

SER

GLN

SER

PHE

ALA

GLN

SER

GLN

ALA

15

TYR

GLN

ALA

SER

ALA

PHE

GLN

ALA

16

ALA

GLN

SER

ALA

GLN

SER

ALA

SER

17

ARG

ALA

Samples:

sample_1: tu81d5, [U-99% 13C; U-99% 15N], 1.0 mM

conditions_1: pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	not available	not available	not available
1H13C_HSQC	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
H(CCCO)NH	not available	not available	not available
CC(CO)NH	not available	not available	not available
3D_1H15N_NOESY	not available	not available	not available
3D_1H13C_NOESY	not available	not available	not available
HSQC_TOCSY	not available	not available	not available

Software:

NMRView v5_2_2, One Moon Scientific, Inc -

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2K3N
GB	ACI23395