BMRB Entry 6846

Title:
NMR structural analysis of Nop10p from Saccharomyces cerevisiae
Deposition date:
2005-09-29
Original release date:
2005-12-28
Authors:
Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.
Citation:

Citation: Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.. "The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs"  Nat. Struct. Mol. Biol. 12, 1101-1107 (2005).
PubMed: 16286935

Assembly members:

Assembly members:
H/ACA ribonucleoprotein complex subunit 3, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H/ACA ribonucleoprotein complex subunit 3: HLMYTLGPDGKRIYTLKKVT ESGEITKSAHPARFSPDDKY SRQRVTLKKRFGLVPGQHHH HHH

Data sets:
Data typeCount
13C chemical shifts234
15N chemical shifts58
1H chemical shifts415

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H/ACA ribonucleoprotein complex subunit 31

Entities:

Entity 1, H/ACA ribonucleoprotein complex subunit 3 63 residues - Formula weight is not available

1   HISLEUMETTYRTHRLEUGLYPROASPGLY
2   LYSARGILETYRTHRLEULYSLYSVALTHR
3   GLUSERGLYGLUILETHRLYSSERALAHIS
4   PROALAARGPHESERPROASPASPLYSTYR
5   SERARGGLNARGVALTHRLEULYSLYSARG
6   PHEGLYLEUVALPROGLYGLNHISHISHIS
7   HISHISHIS

Samples:

sample_1: H/ACA ribonucleoprotein complex subunit 3 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%

sample_2: H/ACA ribonucleoprotein complex subunit 3 1 mM; Sodium Acetate 50 mM; KCl 100 mM; D2O 100%

sample_3: H/ACA ribonucleoprotein complex subunit 3, [U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%

sample_4: H/ACA ribonucleoprotein complex subunit 3, [U-13C; U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

CYANA vv. 2.0, Guntert, P. - refinement

NMRPipe vv. 2.3, Delaglio, F. - processing

SPARKY vv. 3.110, Goddard. T.D. - data analysis

TALOS vv. 2003.027.13.05, Cornilescu, G. - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ GAA23788
EMBL CAY80079
GB AAS56874 AHY77771 EDN62310 EDV09121 EEU04743
PIR S78745
REF NP_058135 XP_011103755
SP Q6Q547
TPG DAA06766
AlphaFold Q6Q547

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks