BMRB Entry 6846

Name: NMR structural analysis of Nop10p from Saccharomyces cerevisiae
Published: 2005-12-28

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PDB ID: 2aqa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6846
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structural analysis of Nop10p from Saccharomyces cerevisiae

Deposition date:

2005-09-29

Original release date:

2005-12-28

Authors:

Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.

Citation:

Citation: Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.. "The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs" Nat. Struct. Mol. Biol. 12, 1101-1107 (2005).
PubMed: 16286935

Assembly members:

Assembly members:
H/ACA ribonucleoprotein complex subunit 3, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: not available Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H/ACA ribonucleoprotein complex subunit 3: HLMYTLGPDGKRIYTLKKVT ESGEITKSAHPARFSPDDKY SRQRVTLKKRFGLVPGQHHH HHH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	234
15N chemical shifts	58
1H chemical shifts	415

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	H/ACA ribonucleoprotein complex subunit 3	1

Entities:

Entity 1, H/ACA ribonucleoprotein complex subunit 3 63 residues - Formula weight is not available

1

HIS

LEU

MET

TYR

THR

LEU

GLY

PRO

ASP

GLY

2

LYS

ARG

ILE

TYR

THR

LEU

LYS

VAL

THR

3

GLU

SER

GLY

GLU

ILE

THR

LYS

SER

ALA

HIS

4

PRO

ALA

ARG

PHE

SER

PRO

ASP

LYS

TYR

5

SER

ARG

GLN

ARG

VAL

THR

LEU

LYS

ARG

6

PHE

GLY

LEU

VAL

PRO

GLY

GLN

HIS

7

HIS

Samples:

sample_1: H/ACA ribonucleoprotein complex subunit 3 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%

sample_2: H/ACA ribonucleoprotein complex subunit 3 1 mM; Sodium Acetate 50 mM; KCl 100 mM; D2O 100%

sample_3: H/ACA ribonucleoprotein complex subunit 3, [U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%

sample_4: H/ACA ribonucleoprotein complex subunit 3, [U-13C; U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available

Software:

CYANA vv. 2.0, Guntert, P. - refinement

NMRPipe vv. 2.3, Delaglio, F. - processing

SPARKY vv. 3.110, Goddard. T.D. - data analysis

TALOS vv. 2003.027.13.05, Cornilescu, G. - data analysis

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1Y2Y 2AQA 3U28 3UAI
DBJ	GAA23788
EMBL	CAY80079
GB	AAS56874 AHY77771 EDN62310 EDV09121 EEU04743
PIR	S78745
REF	NP_058135 XP_011103755
SP	Q6Q547
TPG	DAA06766
AlphaFold	Q6Q547