BMRB Entry 6841

Title:
Solution structure of Calmodulin-like Skin Protein C terminal domain
Deposition date:
2005-09-29
Original release date:
2006-06-27
Authors:
Babini, E.; Bertini, I.; Capozzi, F.; Chirivino, E.; Luchinat, C.
Citation:

Citation: Babini, E.; Bertini, I.; Capozzi, F.; Chirivino, E.; Luchinat, C.. "A Structural and Dynamic Characterization of the EF-Hand Protein CLSP"  Structure 14, 1029-1038 (2006).
PubMed: 16765896

Assembly members:

Assembly members:
Calmodulin-like protein 5, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calmodulin-like protein 5: ARAGLEDLQVAFRAFDQDGD GHITVDELRRAMAGLGQPLP QEELDAMIREADVDQDGRVN YEEFARMLAQE

Data sets:
Data typeCount
15N chemical shifts64
1H chemical shifts427

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin-like protein 51

Entities:

Entity 1, Calmodulin-like protein 5 71 residues - Formula weight is not available

1   ALAARGALAGLYLEUGLUASPLEUGLNVAL
2   ALAPHEARGALAPHEASPGLNASPGLYASP
3   GLYHISILETHRVALASPGLULEUARGARG
4   ALAMETALAGLYLEUGLYGLNPROLEUPRO
5   GLNGLUGLULEUASPALAMETILEARGGLU
6   ALAASPVALASPGLNASPGLYARGVALASN
7   TYRGLUGLUPHEALAARGMETLEUALAGLN
8   GLU

Samples:

sample_1: Calmodulin-like protein 5, [U-15N], 2 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

CARA v1.2 - data analysis

CYANA v1.2 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks