BMRB Entry 6834
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6834
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Citation: Li, M.; Phatnani, H.; Greenleaf, A.; Zhou, P.. "NMR assignment of the SRI domain of human Set2/HYPB." J. Biomol. NMR 36, 5-5 (2006).
PubMed: 16435090
Assembly members:
the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1: GSHMTAEADTSSELAKKSKE
VFRKEMSQFIVQCLNPYRKP
DCKVGRITTTEDFKHLARKL
THGVMNKELKYCKNPEDLEC
NENVKHKTKEYIKKYMQKFG
AVYKPKEDTELE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 381 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 756 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Huntingtin interacting protein B | 1 |
Entities:
Entity 1, Huntingtin interacting protein B 112 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | THR | ALA | GLU | ALA | ASP | THR | ||||
| 2 | SER | SER | GLU | LEU | ALA | LYS | LYS | SER | LYS | GLU | ||||
| 3 | VAL | PHE | ARG | LYS | GLU | MET | SER | GLN | PHE | ILE | ||||
| 4 | VAL | GLN | CYS | LEU | ASN | PRO | TYR | ARG | LYS | PRO | ||||
| 5 | ASP | CYS | LYS | VAL | GLY | ARG | ILE | THR | THR | THR | ||||
| 6 | GLU | ASP | PHE | LYS | HIS | LEU | ALA | ARG | LYS | LEU | ||||
| 7 | THR | HIS | GLY | VAL | MET | ASN | LYS | GLU | LEU | LYS | ||||
| 8 | TYR | CYS | LYS | ASN | PRO | GLU | ASP | LEU | GLU | CYS | ||||
| 9 | ASN | GLU | ASN | VAL | LYS | HIS | LYS | THR | LYS | GLU | ||||
| 10 | TYR | ILE | LYS | LYS | TYR | MET | GLN | LYS | PHE | GLY | ||||
| 11 | ALA | VAL | TYR | LYS | PRO | LYS | GLU | ASP | THR | GLU | ||||
| 12 | LEU | GLU |
Samples:
sample_1: the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1, [U-15N], 2.0 mM; KCl 100 mM; H2O 90%; D2O 10%
sample_2: the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1, [U-95% 13C; U-98% 15N], 2.0 mM; KCl 100 mM; H2O 90%; D2O 10%
sample_3: the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1, [U-15N]-Lys, 2.0 mM; KCl 100 mM; H2O 90%; D2O 10%
sample_4: the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1, [U-95% 13C; U-98% 15N], 2.0 mM; KCl 100 mM; D2O 100%
sample_5: the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1 2.0 mM; KCl 100 mM; D2O 100%
sample_6: the Set2-Rpb1-Interacting domain of Huntingtin interacting protein B isoform 1, [U-10% 13C], 2.0 mM; KCl 100 mM; D2O 100%
sample_cond_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_15N-separated_NOESY | not available | not available | not available |
| 3D_13C-separated_NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HN(CA)CB | not available | not available | not available |
| HN(COCA)CB | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA-J | not available | not available | not available |
| 4D HC(CCO)NH-TOCSY | not available | not available | not available |
| 15N-HSQC | not available | not available | not available |
| high-resolution 13C-HSQC | not available | not available | not available |
| RDC experiment in phage | not available | not available | not available |
Software:
NMRPipe - processing
XEASY v1.2 - data analysis
DYANA v1.5 - structure solution
CYANA v2.0 - structure solution
X-PLOR NIH v2.9.7 - refinement
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB21823 BAD32524 BAG10485 |
| EMBL | CAC28349 CAD28492 |
| GB | AAC26194 AAH31601 AAH59049 AAH90954 AAI17163 |
| REF | NP_001074809 NP_001101659 NP_054878 XP_001113652 XP_001375978 |
| SP | E9Q5F9 Q9BYW2 |
| TPG | DAA16805 |
| AlphaFold | E9Q5F9 Q9BYW2 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks