BMRB Entry 6828

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for MazF(E24A)
Deposition date:
2005-09-19
Original release date:
2006-04-20
Authors:
Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko
Citation:

Citation: Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko. "Characterization of dual substrate binding sites in the homodimeric structure of Escherichia coli mRNA interferase MazF"  J. Mol. Biol. 375, 139-150 (2006).
PubMed: 16413577

Assembly members:

Assembly members:
MazF(E24A), polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts214
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-MazF, subunit 11
2apo-MazF, subunit 21

Entities:

Entity 1, apo-MazF, subunit 1 114 residues - Formula weight is not available

1   GLYSERHISMETVALSERARGTYRVALPRO
2   ASPMETGLYASPLEUILETRPVALASPPHE
3   ASPPROTHRLYSGLYSERALAGLNALAGLY
4   HISARGPROALAVALVALLEUSERPROPHE
5   METTYRASNASNLYSTHRGLYMETCYSLEU
6   CYSVALPROCYSTHRTHRGLNSERLYSGLY
7   TYRPROPHEGLUVALVALLEUSERGLYGLN
8   GLUARGASPGLYVALALALEUALAASPGLN
9   VALLYSSERILEALATRPARGALAARGGLY
10   ALATHRLYSLYSGLYTHRVALALAPROGLU
11   GLULEUGLNLEUILELYSALALYSILEASN
12   VALLEUILEGLY

Samples:

sample_1: MazF(E24A), [U-95% 13C; U-95% 15N], 1.0 ± 0.2 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONNHsample_1not availableconditions_1

Software:

XEASY v1.13 -

NMR spectrometers:

  • Varian Unity/Inova600 600 MHz

Related Database Links:

PDB
DBJ BAA03918 BAB37065 BAE76856 BAG78564 BAI27043
EMBL CAP77215 CAQ33106 CAQ87850 CAQ99710 CAR04292
GB AAA03239 AAA69292 AAC75824 AAG57895 AAZ89538
REF NP_289336 NP_311669 NP_417262 WP_000254731 WP_000254732
SP P0AE70 P0AE71
AlphaFold P0AE71 P0AE70

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks