BMRB Entry 6828

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for MazF(E24A)
Published: 2006-04-20

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6828

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for MazF(E24A)

Deposition date:

2005-09-19

Original release date:

2006-04-20

Authors:

Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko

Citation:

Citation: Li, Guang-Yao; Zhang, Yonglong; Chan, Mitchell; Mal, Tapas; Hoeflich, Klaus; Inouye, Masayori; Ikura, Mitsuhiko. "Characterization of dual substrate binding sites in the homodimeric structure of Escherichia coli mRNA interferase MazF" J. Mol. Biol. 375, 139-150 (2006).
PubMed: 16413577

Assembly members:

Assembly members:
MazF(E24A), polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MazF(E24A): GSHMVSRYVPDMGDLIWVDF DPTKGSAQAGHRPAVVLSPF MYNNKTGMCLCVPCTTQSKG YPFEVVLSGQERDGVALADQ VKSIAWRARGATKKGTVAPE ELQLIKAKINVLIG

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	214
15N chemical shifts	104
1H chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apo-MazF, subunit 1	1
2	apo-MazF, subunit 2	1

Entities:

Entity 1, apo-MazF, subunit 1 114 residues - Formula weight is not available

1

GLY

SER

HIS

MET

VAL

SER

ARG

TYR

VAL

PRO

2

ASP

MET

GLY

ASP

LEU

ILE

TRP

VAL

ASP

PHE

3

ASP

PRO

THR

LYS

GLY

SER

ALA

GLN

ALA

GLY

4

HIS

ARG

PRO

ALA

VAL

LEU

SER

PRO

PHE

5

MET

TYR

ASN

LYS

THR

GLY

MET

CYS

LEU

6

CYS

VAL

PRO

CYS

THR

GLN

SER

LYS

GLY

7

TYR

PRO

PHE

GLU

VAL

LEU

SER

GLY

GLN

8

GLU

ARG

ASP

GLY

VAL

ALA

LEU

ALA

ASP

GLN

9

VAL

LYS

SER

ILE

ALA

TRP

ARG

ALA

ARG

GLY

10

ALA

THR

LYS

GLY

THR

VAL

ALA

PRO

GLU

11

GLU

LEU

GLN

LEU

ILE

LYS

ALA

LYS

ILE

ASN

12

VAL

LEU

ILE

GLY

Samples:

sample_1: MazF(E24A), [U-95% 13C; U-95% 15N], 1.0 ± 0.2 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
CBCACONNH	sample_1	not available	conditions_1

Software:

XEASY v1.13 -

NMR spectrometers:

Varian Unity/Inova600 600 MHz

PDB	1UB4 3NFC
DBJ	BAA03918 BAB37065 BAE76856 BAG78564 BAI27043
EMBL	CAP77215 CAQ33106 CAQ87850 CAQ99710 CAR04292
GB	AAA03239 AAA69292 AAC75824 AAG57895 AAZ89538
REF	NP_289336 NP_311669 NP_417262 WP_000254731 WP_000254732
SP	P0AE70 P0AE71
AlphaFold	P0AE70 P0AE71