BMRB Entry 6816

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6816
MolProbity Validation Chart

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Title:
Solution structure of the C-terminal RpoN domain of aquifex aeolicus SIGMA-54 shows similarity to SIGMA-70
Deposition date:
2005-09-09
Original release date:
2007-01-29
Authors:
Doucleff, M.; Malak, L.; Pelton, J.; Wemmer, D.
Citation:

Citation: Doucleff, M.; Malak, L.; Pelton, J.; Wemmer, D.. "The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70."  J. Biol. Chem. 280, 41530-41536 (2005).
PubMed: 16210314

Assembly members:

Assembly members:
RNA polymerase sigma factor RpoN, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Aquifex aeolicus   Taxonomy ID: 63363   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Aquifex aeolicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA polymerase sigma factor RpoN: TYSLRTFFVRESAEGLTQGE LMKLIKEIVENEDKRKPYSD QEIANILKEKGFKVARRTVA KYREMLGIPSSRERRI

Data sets:
Data typeCount
13C chemical shifts214
15N chemical shifts67
1H chemical shifts434

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA polymerase sigma factor RpoN1

Entities:

Entity 1, RNA polymerase sigma factor RpoN 76 residues - Formula weight is not available

1   THRTYRSERLEUARGTHRPHEPHEVALARG
2   GLUSERALAGLUGLYLEUTHRGLNGLYGLU
3   LEUMETLYSLEUILELYSGLUILEVALGLU
4   ASNGLUASPLYSARGLYSPROTYRSERASP
5   GLNGLUILEALAASNILELEULYSGLULYS
6   GLYPHELYSVALALAARGARGTHRVALALA
7   LYSTYRARGGLUMETLEUGLYILEPROSER
8   SERARGGLUARGARGILE

Samples:

sample_1: RNA polymerase sigma factor RpoN, [U-15N], 0.8 mM; Hepes 50 mM; NaCl 250 mM; EDTA 1 mM; H20 90%; D20 10%

sample_2: RNA polymerase sigma factor RpoN, [U-13C; U-15N], 0.8 mM; Hepes 50 mM; NaCl 250 mM; EDTA 1 mM; H20 90%; D20 10%

sample_3: RNA polymerase sigma factor RpoN, [U-15N], 0.8 mM; Hepes 50 mM; NaCl 250 mM; EDTA 1 mM; H20 5%; D20 95%

sample_4: RNA polymerase sigma factor RpoN, [U-13C; U-15N], 0.8 mM; Hepes 50 mM; NaCl 250 mM; EDTA 1 mM; H20 90%; D20 10%

sample_cond_1: ionic strength: 300 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D N15-seperated TOCSYnot availablenot availablenot available
HNCA-Jnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
2D C13-HSQCnot availablenot availablenot available

Software:

NMRPipe v2.3 - processing

NMRView v5.0.4 - data analysis

DYANA v1.5 - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15083
PDB
GB AAC06814
REF NP_213418 WP_010880356

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks