BMRB Entry 6815

Title:
NMR structure of Retrocyclin-2 in SDS   PubMed: 17685559
Deposition date:
2005-09-09
Original release date:
2008-06-24
Authors:
Daly, N.; Chen, Y.-K.; Rosengren, K.; Marx, U.; Phillips, M.; Waring, A.; Wang, W.; Lehrer, R.; Craik, D.
Citation:

Citation: Daly, N.; Chen, Y.-K.; Rosengren, K.; Marx, U.; Phillips, M.; Waring, A.; Wang, W.; Lehrer, R.; Craik, D.. "Retrocyclin-2: structural analysis of a potent anti-HIV theta-defensin"  Biochemistry 46, 9920-9928 (2007).

Assembly members:

Assembly members:
Retrocyclin-2, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Retrocyclin-2: RRICRCICGRGICRCICG

Data sets:
Data typeCount
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Retrocyclin-21

Entities:

Entity 1, Retrocyclin-2 18 residues - Formula weight is not available

1   ARGARGILECYSARGCYSILECYSGLYARG
2   GLYILECYSARGCYSILECYSGLY

Samples:

sample_1: Retrocyclin-2 0.6 mM; SDS 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 1 M; pH: 3.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

XEASY v1.3.7 - data analysis

DYANA v1.5 - structure solution

CNS v1.1 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 600 MHz