BMRB Entry 6810

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6810
MolProbity Validation Chart

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Title:
Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the last 250 residues in Escherichia coli RcsC, including the phosphoreceiver domain.
Deposition date:
2005-09-07
Original release date:
2006-10-19
Authors:
Rogov, Vladimir; Loehr, Frank; Rogova, Natalia; Klammt, Christian; Koglin, Alexander; Bernhard, Frank; Doetsch, Volker
Citation:

Citation: Rogov, Vladimir; Loehr, Frank; Rogova, Natalia; Klammt, Christian; Koglin, Alexander; Bernhard, Frank; Doetsch, Volker. "Letter to the Editor : NMR assignment of (1)H, (13)C and (15)N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain"  J. Biomol. NMR 38, 165-165 (2006).
PubMed: 17036162

Assembly members:

Assembly members:
C'-RcsC, polymer, 254 residues, 27908 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: monera   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pQE60

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C'-RcsC: MGGSGVEGLSGKRCWLAVRN ASLCQFLETSLQRSGIVVTT YEGQEPTPEDVLITDEVVSK KWQGRAVVTFCRRHIGIPLE KAPGEWVHSVAAPHELPALL ARIYLIEMESDDPANALPST DKAVSDNDDMMILVVDDHPI NRRLLADQLGSLGYQCKTAN DGVDALNVLSKNHIDIVLSD VNMPNMDGYRLTQRIRQLGL TLPVIGVTANALAEEKQRCL ESGMDSCLSKPVTLDVIKQT LTLYAERVRKSRDS

Data sets:
Data typeCount
13C chemical shifts1068
15N chemical shifts241
1H chemical shifts1717

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C'-RcsC1

Entities:

Entity 1, C'-RcsC 254 residues - 27908 Da.

Residues 5-254 correspond to the E.coli RcsC residues 700-949

1   METGLYGLYSERGLYVALGLUGLYLEUSER
2   GLYLYSARGCYSTRPLEUALAVALARGASN
3   ALASERLEUCYSGLNPHELEUGLUTHRSER
4   LEUGLNARGSERGLYILEVALVALTHRTHR
5   TYRGLUGLYGLNGLUPROTHRPROGLUASP
6   VALLEUILETHRASPGLUVALVALSERLYS
7   LYSTRPGLNGLYARGALAVALVALTHRPHE
8   CYSARGARGHISILEGLYILEPROLEUGLU
9   LYSALAPROGLYGLUTRPVALHISSERVAL
10   ALAALAPROHISGLULEUPROALALEULEU
11   ALAARGILETYRLEUILEGLUMETGLUSER
12   ASPASPPROALAASNALALEUPROSERTHR
13   ASPLYSALAVALSERASPASNASPASPMET
14   METILELEUVALVALASPASPHISPROILE
15   ASNARGARGLEULEUALAASPGLNLEUGLY
16   SERLEUGLYTYRGLNCYSLYSTHRALAASN
17   ASPGLYVALASPALALEUASNVALLEUSER
18   LYSASNHISILEASPILEVALLEUSERASP
19   VALASNMETPROASNMETASPGLYTYRARG
20   LEUTHRGLNARGILEARGGLNLEUGLYLEU
21   THRLEUPROVALILEGLYVALTHRALAASN
22   ALALEUALAGLUGLULYSGLNARGCYSLEU
23   GLUSERGLYMETASPSERCYSLEUSERLYS
24   PROVALTHRLEUASPVALILELYSGLNTHR
25   LEUTHRLEUTYRALAGLUARGVALARGLYS
26   SERARGASPSER

Samples:

sample_1: C'-RcsC, [U-95% 15N], 1 ± 0.1 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_2: C'-RcsC, [U-95% 13C; U-95% 15N], 1 ± 0.1 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_3: C'-RcsC, [95% 15N]-ALA, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_4: C'-RcsC, [95% 15N]-ASN, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_5: C'-RcsC, [95% 15N]-ASP, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_6: C'-RcsC, [95% 15N]-ARG, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_7: C'-RcsC, [95% 15N]-CYS, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_8: C'-RcsC, [95% 15N]-GLY, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_9: C'-RcsC, [95% 15N]-SER, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

sample_10: C'-RcsC, [95% 15N]-THR, 0.25 ± 0.05 mM; Tris-HCl 33 ± 1 mM; NaCl 125 ± 5 mM; TCEP 1 mM

conditions_1: pH: 7.5; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
1H15N_TOCSYnot availablenot availableconditions_1
1H13C15N_(15N_1H)-TROSY-HNCOnot availablenot availableconditions_1
1H13C15N_(15N_1H)-TROSY-HNCACBnot availablenot availableconditions_1
1H13C15N_(HCA)CO(CA)NHnot availablenot availableconditions_1
1H13C15N_H(CC)(CO)NH-TOCSYnot availablenot availableconditions_1
1H13C15N_(H)CC(CO)NH-TOCSYnot availablenot availableconditions_1
1H13C15N_H(CDCGCBCACO)NHnot availablenot availableconditions_1
1H13C15N_(H)CD(CGCBCACO)NHnot availablenot availableconditions_1
1H13C15N_HC(CN)H-TOCSY_TRPnot availablenot availableconditions_1

Software:

AURELIA v2.7.5 -

SPARKY v3.110 -

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAB36530 BAG78010 BAI26357 BAI31465 BAI36786
EMBL CAP76721 CAQ32621 CAQ99146 CAR03647 CAR08865
GB AAA24503 AAA24505 AAC75278 AAG57353 AAN43820
REF NP_288798 NP_311134 NP_416722 NP_708113 NP_754647
SP P0DMC5 P0DMC6
AlphaFold P0DMC5 P0DMC6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks