BMRB Entry 6787

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6787

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for rabphilin C2A domain
Deposition date:
2005-08-23
Original release date:
2006-04-14
Authors:
Montaville, Pierre; Kim, Hai-Young; Vijayan, Vinesh; Becker, Stefan; Zweckstetter, Markus
Citation:

Citation: Montaville, Pierre; Kim, Hai-Young; Vijayan, Vinesh; Becker, Stefan; Zweckstetter, Markus. "1H, 15N, and 13C Resonance Assignment of the C2A Domain of Rabphilin3A"  J. Biomol. NMR 36, 20-20 (2006).
PubMed: 16609836

Assembly members:

Assembly members:
C2A domain, polymer, 140 residues, 16336.42 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2A domain: EANSYDSDEATTLGALEFSL LYDQDNSNLQCTIIRAKGLK PMDSNGLADPYVKLHLLPGA SKSNKLRTKTLRNTRNPVWN ETLQYHGITEEDMQRKTLRI SVCDEDKFGHNEFIGETRFS LKKLKANQRKNFNICLERVI

Data sets:
Data typeCount
13C chemical shifts553
15N chemical shifts153
1H chemical shifts968

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C2A1
2CALCIUM (II) ION 12
3CALCIUM (II) ION 22
4CALCIUM (II) ION 32

Entities:

Entity 1, C2A 140 residues - 16336.42 Da.

1   GLUALAASNSERTYRASPSERASPGLUALA
2   THRTHRLEUGLYALALEUGLUPHESERLEU
3   LEUTYRASPGLNASPASNSERASNLEUGLN
4   CYSTHRILEILEARGALALYSGLYLEULYS
5   PROMETASPSERASNGLYLEUALAASPPRO
6   TYRVALLYSLEUHISLEULEUPROGLYALA
7   SERLYSSERASNLYSLEUARGTHRLYSTHR
8   LEUARGASNTHRARGASNPROVALTRPASN
9   GLUTHRLEUGLNTYRHISGLYILETHRGLU
10   GLUASPMETGLNARGLYSTHRLEUARGILE
11   SERVALCYSASPGLUASPLYSPHEGLYHIS
12   ASNGLUPHEILEGLYGLUTHRARGPHESER
13   LEULYSLYSLEULYSALAASNGLNARGLYS
14   ASNPHEASNILECYSLEUGLUARGVALILE

Entity 2, CALCIUM (II) ION 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: rabphilin C2A domain, [U-13C; U-15N], 1 ± 0.1 mM; Na acetate 20 mM; NaCl 150 mM; CaCl2 20 mM; DTT 1 mM

conditions_1: ionic strength: 150 mM; pH: 5.0; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D [15N, 1H] HSQCsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
3D HN(CO)CAsample_1not availableconditions_1
3D CBCA(CO)NHsample_1not availableconditions_1
3D HNCACBsample_1not availableconditions_1
3D C(CO)NNHsample_1not availableconditions_1
3D H(CCO)NNHsample_1not availableconditions_1
3D HCCH-TOCSYsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D [15N, 1H] NOESY-HSQCsample_1not availableconditions_1
3D [13C, 1H] NOESY-HMQCsample_1not availableconditions_1
2D (Hb)Cb(CgCd)Hdsample_1not availableconditions_1
2D (Hb)Cb(CgCdCe)Hesample_1not availableconditions_1

Software:

MARS - semi automated backbone assignment

NMR spectrometers:

  • Bruker . 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks