BMRB Entry 6782
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6782
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ramelot, Theresa; Xiao, Rong; Montelione, Gaetano; Kennedy, Michael. "Solution NMR Structure of E. coli YbiA. Northeast Structural Genomics Target ET24." The BMRB entry is the only known published source for the data..
Assembly members:
YbiA, polymer, 168 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Eschericia coli
Experimental source: Production method: recombinant technology Vector: pet21
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 737 |
| 15N chemical shifts | 170 |
| 1H chemical shifts | 1167 |
| coupling constants | 44 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YbiA | 1 |
Entities:
Entity 1, YbiA 168 residues - Formula weight is not available
| 1 | MET | PRO | VAL | ARG | ALA | GLN | ARG | ILE | GLN | HIS | ||||
| 2 | VAL | MET | GLN | ASP | THR | ILE | ILE | ASN | PHE | TYR | ||||
| 3 | SER | THR | SER | ASP | ASP | TYR | GLY | ASP | PHE | SER | ||||
| 4 | ASN | PHE | ALA | ALA | TRP | PRO | ILE | LYS | VAL | ASP | ||||
| 5 | GLY | LYS | THR | TRP | PRO | THR | SER | GLU | HIS | TYR | ||||
| 6 | PHE | GLN | ALA | GLN | LYS | PHE | LEU | ASP | GLU | LYS | ||||
| 7 | TYR | ARG | GLU | GLU | ILE | ARG | ARG | VAL | SER | SER | ||||
| 8 | PRO | MET | VAL | ALA | ALA | ARG | MET | GLY | ARG | ASP | ||||
| 9 | ARG | SER | LYS | PRO | LEU | ARG | LYS | ASN | TRP | GLU | ||||
| 10 | SER | VAL | LYS | GLU | GLN | VAL | MET | ARG | LYS | ALA | ||||
| 11 | LEU | ARG | ALA | LYS | PHE | GLU | GLN | HIS | ALA | GLU | ||||
| 12 | LEU | ARG | ALA | LEU | LEU | LEU | ALA | THR | ALA | PRO | ||||
| 13 | ALA | LYS | LEU | VAL | GLU | HIS | THR | GLU | ASN | ASP | ||||
| 14 | ALA | TYR | TRP | GLY | ASP | GLY | GLY | HIS | GLY | LYS | ||||
| 15 | GLY | LYS | ASN | ARG | LEU | GLY | TYR | LEU | LEU | MET | ||||
| 16 | GLU | LEU | ARG | GLU | GLN | LEU | ALA | ILE | GLU | LYS | ||||
| 17 | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: YbiA, [U-15N; U-13C], 1 ± 0.2 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM; NaN3 0.02%; D2O 10%
sample_2: YbiA, [U-15N; U-5% 13C], 1 ± 0.2 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM; NaN3 0.02%; D2O 10%
sample_3: YbiA, [U-15N; U-13C], 1 ± 0.2 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM; NaN3 0.02%; D2O 100%
conditions_1: pH: 6.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C NOESY (arom) | sample_1 | isotropic | conditions_1 |
| CBCACONH | sample_1 | isotropic | conditions_1 |
| CCONH | sample_1 | isotropic | conditions_1 |
| CCH-TOCSY | sample_1 | isotropic | conditions_1 |
| 1H13C HSQC (aliph) | sample_1 | isotropic | conditions_1 |
| 1H13C HSQC (arom) | sample_1 | isotropic | conditions_1 |
| HBCBCGCDCEHE | sample_1 | isotropic | conditions_1 |
| HBCBCGCDHD | sample_1 | isotropic | conditions_1 |
| HCCONH | sample_1 | isotropic | conditions_1 |
| HCCH-COSY | sample_1 | isotropic | conditions_1 |
| HCCH-TOCSY | sample_1 | isotropic | conditions_1 |
| HNCA | sample_1 | isotropic | conditions_1 |
| HNCACB | sample_1 | isotropic | conditions_1 |
| HNCO | sample_1 | isotropic | conditions_1 |
| HNCOCA | sample_1 | isotropic | conditions_1 |
| 1H15N HSQC (long) | sample_1 | isotropic | conditions_1 |
| 1H15N HSQC (NH2 only) | sample_1 | isotropic | conditions_1 |
| 1H15N HSQC (sw1) | sample_1 | isotropic | conditions_1 |
| 1H13C HSQC CT (arom) | sample_1 | isotropic | conditions_1 |
| 3D 13C NOESY (aliph) | sample_1 | isotropic | conditions_1 |
| 3D 15N NOESY | sample_1 | isotropic | conditions_1 |
| 1H15N HSQC | sample_1 | isotropic | conditions_1 |
| 1H13C HSQC (long) | sample_2 | isotropic | conditions_1 |
| HNHA | sample_2 | isotropic | conditions_1 |
| 1H15N HSQC | sample_2 | isotropic | conditions_1 |
| 1H15N HSQC (15 min) | sample_3 | isotropic | conditions_1 |
| 1H15N HSQC (22 days) | sample_3 | isotropic | conditions_1 |
| 3D 13C NOESY (aliph) | sample_3 | isotropic | conditions_1 |
| 4D 1H13C-1H13C 4D noesy | sample_3 | isotropic | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity Inova 600 MHz
- Varian Unity Inova 750 MHz
- Varian Unity Inova 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA35458 BAG76378 BAI24243 BAI29685 BAI34816 |
| EMBL | CAQ31299 CAQ89811 CAQ97701 CAR12151 CAU96708 |
| GB | AAA53654 AAC73885 AAZ87525 ABB65362 ABV05209 |
| REF | NP_415319 WP_001145123 WP_001145126 WP_001145127 WP_001145128 |
| SP | P30176 Q323Z6 Q3Z3Y7 |
| AlphaFold | P30176 Q323Z6 Q3Z3Y7 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks