BMRB Entry 6780

Name: NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae
Published: 2005-12-20

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PDB ID: 2out
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6780
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae

Deposition date:

2005-08-17

Original release date:

2005-12-20

Authors:

Sari, Nese; Doseeva, Victoria; Subarian, Karen; Herzberg, Osnat; Orban, John

Citation:

Citation: Sari, Nese; Yeh, Deok; Doseeva, Victoria; Surabian, Karen; Herzberg, Osnat; Orban, John. "NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae" J. Biomol. NMR 33, 281-281 (2005).
PubMed: 16341755

Assembly members:

Assembly members:
hi1506, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Haemophilus influenza Taxonomy ID: 727 Superkingdom: Bacteria Kingdom: not available Genus/species: Haemophilus influenzae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hi1506: GSHMDKTFCVVVQNRIKEGY RRAGFSFHLGDNSLAAVSES QLAQLKADPRLVVQITETGS QEGGEGLSKEPAGSDEQKQL RADPPSTDLNTFTVEQLKAQ LTERGITFKQSATKAELIAL FAPADGEKSEA

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	520
15N chemical shifts	129
1H chemical shifts	790

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hi1506	1

Entities:

Entity 1, hi1506 131 residues - Formula weight is not available

1

GLY

SER

HIS

MET

ASP

LYS

THR

PHE

CYS

VAL

2

VAL

GLN

ASN

ARG

ILE

LYS

GLU

GLY

TYR

3

ARG

ALA

GLY

PHE

SER

PHE

HIS

LEU

GLY

4

ASP

ASN

SER

LEU

ALA

VAL

SER

GLU

SER

5

GLN

LEU

ALA

GLN

LEU

LYS

ALA

ASP

PRO

ARG

6

LEU

VAL

GLN

ILE

THR

GLU

THR

GLY

SER

7

GLN

GLU

GLY

GLU

GLY

LEU

SER

LYS

GLU

8

PRO

ALA

GLY

SER

ASP

GLU

GLN

LYS

GLN

LEU

9

ARG

ALA

ASP

PRO

SER

THR

ASP

LEU

ASN

10

THR

PHE

THR

VAL

GLU

GLN

LEU

LYS

ALA

GLN

11

LEU

THR

GLU

ARG

GLY

ILE

THR

PHE

LYS

GLN

12

SER

ALA

THR

LYS

ALA

GLU

LEU

ILE

ALA

LEU

13

PHE

ALA

PRO

ALA

ASP

GLY

GLU

LYS

SER

GLU

14

ALA

Samples:

sample_1: hi1506, [U-13C; U-15N], 1.0 ± 0.1 mM; potassium phosphate 50 mM; NaCl 100 mM; DTT 1 mM; D2O 90%; H2O 10%

conditions_1: ionic strength: 100 mM; pH: 7; pressure: 760 mmHg; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15n-hsqc	sample_1	not available	conditions_1
13c-hsqc	sample_1	not available	conditions_1
hbcbhd	sample_1	not available	conditions_1
hbcbhdhe	sample_1	not available	conditions_1
hncacb	sample_1	not available	conditions_1
cbcaconh	sample_1	not available	conditions_1
hnco	sample_1	not available	conditions_1
hbhaconh	sample_1	not available	conditions_1
hcconh	sample_1	not available	conditions_1
cconh	sample_1	not available	conditions_1
15n-noesy-hsqc	sample_1	not available	conditions_1
13c-noesy-hsqc	sample_1	not available	conditions_1

Software:

SPARKY v2.0 - manual peak assignments

NMR spectrometers:

Bruker AVANCE 600 MHz

PDB	2OUT
GB	AAC23165 EEW76901
REF	WP_005693527
SP	P44228
AlphaFold	P44228