BMRB Entry 6771

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PDB ID: 2a63
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6771
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of a "retroevolved" lambda Cro monomer reveals the basis for increased monomer stability and reduced dimerization

Deposition date:

2005-08-11

Original release date:

2006-06-26

Authors:

Newlove, T.; LeFevre, K.; Cordes, M.

Citation:

Citation: Newlove, T.; Atkinson, K.; Van Dorn, L.; Cordes, M.. "A trade between similar but nonequivalent intrasubunit and intersubunit contacts in cro dimer evolution" Biochemistry 45, 6379-6391 (2006).
PubMed: 16700549

Assembly members:

Assembly members:
Regulatory protein cro, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Enterobacteria phage lambda Taxonomy ID: 10710 Superkingdom: Viruses Kingdom: not available Genus/species: Lambda-like viruses Bacteriophage lambda

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Regulatory protein cro: MEQRITLKDYAMRFGQTKTA KDLGVQQSAINKWIHAGRKI FLTINADGSVYAEEVKPDPS NKKTTA

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	74
1H chemical shifts	482

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Regulatory protein cro	1

Entities:

Entity 1, Regulatory protein cro 66 residues - Formula weight is not available

1

MET

GLU

GLN

ARG

ILE

THR

LEU

LYS

ASP

TYR

2

ALA

MET

ARG

PHE

GLY

GLN

THR

LYS

THR

ALA

3

LYS

ASP

LEU

GLY

VAL

GLN

SER

ALA

ILE

4

ASN

LYS

TRP

ILE

HIS

ALA

GLY

ARG

LYS

ILE

5

PHE

LEU

THR

ILE

ASN

ALA

ASP

GLY

SER

VAL

6

TYR

ALA

GLU

VAL

LYS

PRO

ASP

PRO

SER

7

ASN

LYS

THR

ALA

Samples:

sample_1: Regulatory protein cro, [U-13C], 2.5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; H2O 90%; D2O 10%

sample_2: Regulatory protein cro, [U-15N], 5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; H2O 90%; D2O 10%

sample_3: Regulatory protein cro 5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; H2O 90%; D2O 10%

sample_4: Regulatory protein cro, [U-15N], 5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; D2O 100%

sample_cond_1: pH: 5.3; pressure: 1 atm; temperature: 293 K

sample_cond_2: pH: 6.1; pressure: 1 atm; temperature: 293 K

sample_cond_3: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available
HNHB	not available	not available	not available
HSQC (amide hydrogen exchange)	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available
2D NOESY	not available	not available	not available

Software:

xwinnmr v3.1 - collection

NMRPipe v1.8 - processing

NMRView v4.1.3 - data analysis

CNS v1.1 - refinement, structure solution

NMR spectrometers:

Bruker DRX 600 MHz