BMRB Entry 6762
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6762
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Elantak, Latifa; Ansaldi, Mireille; Guerlesquin, Fran?oise; Mejean, Vincent; Morelli, Xavier. "Structural and Genetic analyses reveal a key role in prophage excision for the
TorI response regulator inhibitor" J. Biol. Chem. 280, 36802-36808 (2005).
PubMed: 16079126
Assembly members:
prophage excisionase, polymer, 66 residues, 7677 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
prophage excisionase: MQHELQPDSLVDLKFIMADT
GFGKTFIYDRIKSGDLPKAK
VIHGRARWLYRDHCEFKNKL
LSRANG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 890 |
| 13C chemical shifts | 222 |
| 15N chemical shifts | 124 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tor inhibition protein | 1 |
Entities:
Entity 1, Tor inhibition protein 66 residues - 7677 Da.
| 1 | MET | GLN | HIS | GLU | LEU | GLN | PRO | ASP | SER | LEU | ||||
| 2 | VAL | ASP | LEU | LYS | PHE | ILE | MET | ALA | ASP | THR | ||||
| 3 | GLY | PHE | GLY | LYS | THR | PHE | ILE | TYR | ASP | ARG | ||||
| 4 | ILE | LYS | SER | GLY | ASP | LEU | PRO | LYS | ALA | LYS | ||||
| 5 | VAL | ILE | HIS | GLY | ARG | ALA | ARG | TRP | LEU | TYR | ||||
| 6 | ARG | ASP | HIS | CYS | GLU | PHE | LYS | ASN | LYS | LEU | ||||
| 7 | LEU | SER | ARG | ALA | ASN | GLY |
Samples:
sample_1: prophage excisionase, [U-95% 13C; U-95% 15N], 1.5 mM; phosphate buffer 50 mM; H20 90%; D20 10%
sample_2: prophage excisionase 1.5 mM; phosphate buffer 50 mM; H20 90%; D20 10%
sample_3: prophage excisionase, [U-95% 15N], 1.5 mM; phosphate buffer 50 mM; H20 90%; D20 10%
sample_4: prophage excisionase, [U-95% 13C], 1.5 mM; phosphate buffer 50 mM; H20 90%; D20 10%
Condition_1: pH: 5.9; temperature: 278 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | Condition_1 |
| 3D-HNCO | not available | not available | Condition_1 |
| 3D-HNCA | not available | not available | Condition_1 |
| CBCA(CO)NH | not available | not available | Condition_1 |
| HN(CO)CA | not available | not available | Condition_1 |
| HCCH-TOCSY | not available | not available | Condition_1 |
| 2D NOESY | not available | not available | Condition_1 |
| 2D TOCSY | not available | not available | Condition_1 |
| 3D 13C-separated NOESY | not available | not available | Condition_1 |
| HNHA | not available | not available | Condition_1 |
Software:
XWINNMR v3.1 - Processing
FELIX v2002 - data analysis
CNS vARIA 1.2 - structure solution, refinement
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE76704 BAI26599 BAJ44158 BAL39139 |
| EMBL | CAR03832 CAS10101 CAU98520 CCK47653 CCQ29767 |
| GB | AAK28851 AAQ12208 AAY17072 ABD18695 ABE08160 |
| REF | NP_112036 NP_958194 WP_001163427 WP_001163428 WP_001163429 |
| SP | Q1R904 Q2EES9 Q716F7 Q9AZ38 |
| AlphaFold | Q1R904 Q2EES9 Q716F7 Q9AZ38 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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