BMRB Entry 6758

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6758

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Title:
NMR assignment of the E.coli cytolethal distending toxin CdtB-II subunit
Deposition date:
2005-08-02
Original release date:
2006-03-10
Authors:
Villar-Lecumberri, M.; Potter, B.; Wang, Zhonghua; Dreyfus, L.; Laity, J.
Citation:

Citation: Villar-Lecumberri, Maria; Potter, Belinda; Wang, Zhonghua; Dreyfus, Lawrence; Laity, John. "NMR Assignment of the E. coli-II Cytolethal Distending Toxin CdtB Subunit"  J. Biomol. NMR 36, 14-14 (2006).
PubMed: 16456702

Assembly members:

Assembly members:
EcCdtB-II, polymer, 262 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EcCdtB-II: MAHHHHHHVGTDLTDFRVAT WNLQGASATTESKWNINVRQ LISGENAVDILAVQEAGSPP STAVDTGRVIPSPGIPVREL IWNLSTNSRPQQVYIYFSAV DALGGRVNLALVSNRRADEV FVLSPVRQGGRPLLGIRIGN DAFFTAHAIAMRNNDAPALV EEVYNFFRDSRDPVHQALNW MILGDFNREPADLEMNLTVP VRRASEIISPAAATQTSQRT LDYAVAGNSVAFRPSPLQAG IVYGARRTQISSDHFPVGVS RR

Data sets:
Data typeCount
13C chemical shifts614
15N chemical shifts200
1H chemical shifts204
heteronuclear NOE values169
T1 relaxation values178
T2 relaxation values180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EcCdtB-II1

Entities:

Entity 1, EcCdtB-II 262 residues - Formula weight is not available

The recombinant protein contains the following His-tag presequence: MAHHHHHHVGT. This presequence is not included in the deposited data.

1   METALAHISHISHISHISHISHISVALGLY
2   THRASPLEUTHRASPPHEARGVALALATHR
3   TRPASNLEUGLNGLYALASERALATHRTHR
4   GLUSERLYSTRPASNILEASNVALARGGLN
5   LEUILESERGLYGLUASNALAVALASPILE
6   LEUALAVALGLNGLUALAGLYSERPROPRO
7   SERTHRALAVALASPTHRGLYARGVALILE
8   PROSERPROGLYILEPROVALARGGLULEU
9   ILETRPASNLEUSERTHRASNSERARGPRO
10   GLNGLNVALTYRILETYRPHESERALAVAL
11   ASPALALEUGLYGLYARGVALASNLEUALA
12   LEUVALSERASNARGARGALAASPGLUVAL
13   PHEVALLEUSERPROVALARGGLNGLYGLY
14   ARGPROLEULEUGLYILEARGILEGLYASN
15   ASPALAPHEPHETHRALAHISALAILEALA
16   METARGASNASNASPALAPROALALEUVAL
17   GLUGLUVALTYRASNPHEPHEARGASPSER
18   ARGASPPROVALHISGLNALALEUASNTRP
19   METILELEUGLYASPPHEASNARGGLUPRO
20   ALAASPLEUGLUMETASNLEUTHRVALPRO
21   VALARGARGALASERGLUILEILESERPRO
22   ALAALAALATHRGLNTHRSERGLNARGTHR
23   LEUASPTYRALAVALALAGLYASNSERVAL
24   ALAPHEARGPROSERPROLEUGLNALAGLY
25   ILEVALTYRGLYALAARGARGTHRGLNILE
26   SERSERASPHISPHEPROVALGLYVALSER
27   ARGARG

Samples:

sample_1: EcCdtB-II, [U-13C; U-15N; U-2H], 0.3 – 0.5 mM; Sodium phosphate 20 mM; Sodium Chloride 50 mM; DSS 0.2 mM; D2O 5 % (v/v); Sodium azide 0.02 % (w/v)

conditions_1: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D_1H-15N_HSQCsample_1not availableconditions_1
3D_HNCACBsample_1not availableconditions_1
3D_CBCA(CO)NHsample_1not availableconditions_1
3D_HNCAsample_1not availableconditions_1
3D_(HCA)CO(CA)NHsample_1not availableconditions_1
3D_HNCOsample_1not availableconditions_1
2D_15N-{1H}-HNOEsample_1not availableconditions_1
2D_1H-15N_HSQC_(T1_flag)sample_1not availableconditions_1
2D_1H-15N_HSQC_(T2_flag)sample_1not availableconditions_1

Software:

NMRView v5.0.4 - Data analysis

NMRPipe v97.027.12.56 - Raw spectral data processing

CurveFit v1.30, . - Used to calculate RelaxT times

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAH72965 BAL47193 BAL47198 BAL47199 BAL47200
GB AAA18786 ACH53455 ACH53456 ACH53457 ADD59823
SP Q46669
AlphaFold Q46669

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks