BMRB Entry 6757
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6757
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Sapay, Nicolas; Montserret, Roland; Chipot, Christophe; Brass, Volker; Moradpour, Darius; Deleage, Gilbert; Penin, Francois. "NMR Structure and Molecular Dynamics of the In-Plane Membrane Anchor of
Nonstructural Protein 5A from Bovine Viral Diarrhea Virus" Biochemistry 45, 2221-2233 (2006).
PubMed: 16475810
Assembly members:
BVDV NS5A[1-28], polymer, 28 residues, 3199.79 Da.
Natural source: Common Name: Bovine Viral Diarrhea Virus Taxonomy ID: 268305 Superkingdom: Viruses Kingdom: Not applicable Genus/species: Pestivirus Bovine viral diarrhea virus 1
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
BVDV NS5A[1-28]: SGNYVLDLIYSLHKQINRGL
KKIVLGWA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 203 |
| 1H chemical shifts | 452 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | membrane anchor domain of BVDV NS5A | 1 |
Entities:
Entity 1, membrane anchor domain of BVDV NS5A 28 residues - 3199.79 Da.
| 1 | SER | GLY | ASN | TYR | VAL | LEU | ASP | LEU | ILE | TYR | ||||
| 2 | SER | LEU | HIS | LYS | GLN | ILE | ASN | ARG | GLY | LEU | ||||
| 3 | LYS | LYS | ILE | VAL | LEU | GLY | TRP | ALA |
Samples:
TFE_50%: BVDV NS5A[1-28] 2 ± 0.1 mM; 2,2,2-TriFluoroEthanol-d2, TFE-d2 (>99%), 50 ± 1 % v/v; water 50 ± 1 % v/v; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.01 mM
SDS_100_mM: BVDV NS5A[1-28] 2 ± 0.1 mM; Sodium Dodecyl Sulfate (SDS)-d25, SDS-d25 (>98%), 100 ± 1 mM; water 100 ± 1 % v/v; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.01 mM
303K: pH: 4.5; temperature: 303 K
313K: pH: 4.5; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY in TFE 50% | not available | not available | not available |
| 1H_13C_HSQC in TFE 50% | not available | not available | not available |
| NOESY in TFE 50% | not available | not available | not available |
| TOCSY in SDS 100 mM | not available | not available | not available |
| 1H_13C_HSQC in SDS 100 mM | not available | not available | not available |
| NOESY in SDS 100 mM | not available | not available | not available |
Software:
SPARKY v3.110, T. D. Goddard and D. G. Kneller - Chemical shift assignment
VNMR v6.1C, Varian - Spectra processing
NMR spectrometers:
- Varian Unity-plus 500 MHz