BMRB Entry 6752

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6752
MolProbity Validation Chart

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Title:
Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for the PH-PDZ tandem of alpha syntrophin
Deposition date:
2005-07-27
Original release date:
2007-01-29
Authors:
Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie
Citation:

Citation: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie. "Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin"  EMBO J. 24, 3985-3995 (2005).
PubMed: 16252003

Assembly members:

Assembly members:
PH-PDZ tandem, polymer, 263 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH-PDZ tandem: ASGRRAPRTGLLELRCGAGS GAGGERWQRVLLSLAEDALT VSPADGEPGPEPEPAQLNGA AEPGAAPPQLPEALLLQRRR VTVRKADAGGLGISIKGGRE NKMPILISKIFKGLAADQTE ALFVGDAILSVNGEDLSSAT HDEAVQALKKTGKEVVLEVK YMKEVSPYFKNSAGGTSVGW DSPPASPLQRQPSSPGPQPR NLSEAKHVSLKMAYVSRRCT PTDPEPRYLEICAADGQDAV FLRAKDEASARSWAGAIQAQ IGT

Data sets:
Data typeCount
13C chemical shifts647
15N chemical shifts245
1H chemical shifts1650

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PH-PDZ tandem1

Entities:

Entity 1, PH-PDZ tandem 263 residues - Formula weight is not available

1   ALASERGLYARGARGALAPROARGTHRGLY
2   LEULEUGLULEUARGCYSGLYALAGLYSER
3   GLYALAGLYGLYGLUARGTRPGLNARGVAL
4   LEULEUSERLEUALAGLUASPALALEUTHR
5   VALSERPROALAASPGLYGLUPROGLYPRO
6   GLUPROGLUPROALAGLNLEUASNGLYALA
7   ALAGLUPROGLYALAALAPROPROGLNLEU
8   PROGLUALALEULEULEUGLNARGARGARG
9   VALTHRVALARGLYSALAASPALAGLYGLY
10   LEUGLYILESERILELYSGLYGLYARGGLU
11   ASNLYSMETPROILELEUILESERLYSILE
12   PHELYSGLYLEUALAALAASPGLNTHRGLU
13   ALALEUPHEVALGLYASPALAILELEUSER
14   VALASNGLYGLUASPLEUSERSERALATHR
15   HISASPGLUALAVALGLNALALEULYSLYS
16   THRGLYLYSGLUVALVALLEUGLUVALLYS
17   TYRMETLYSGLUVALSERPROTYRPHELYS
18   ASNSERALAGLYGLYTHRSERVALGLYTRP
19   ASPSERPROPROALASERPROLEUGLNARG
20   GLNPROSERSERPROGLYPROGLNPROARG
21   ASNLEUSERGLUALALYSHISVALSERLEU
22   LYSMETALATYRVALSERARGARGCYSTHR
23   PROTHRASPPROGLUPROARGTYRLEUGLU
24   ILECYSALAALAASPGLYGLNASPALAVAL
25   PHELEUARGALALYSASPGLUALASERALA
26   ARGSERTRPALAGLYALAILEGLNALAGLN
27   ILEGLYTHR

Samples:

sample_1: PH-PDZ tandem, [U-13C; U-15N], 1 mM

sample_2: PH-PDZ tandem, [U-15N], 1 mM

conditions_1: pH: 7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15C-separated NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz

Related Database Links:

PDB
DBJ BAE23334
GB AAC52119 AAH18546 AAI69101 EDL06068 EDL06069
PRF 2208451B
REF NP_001094371 NP_033254 XP_006235388 XP_007640040
SP Q61234
AlphaFold Q61234

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks