BMRB Entry 6748

Name: NMR assignments of the Ki67FHA/hNIFK(226-269)3P complex
Published: 2007-01-29

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PDB ID: 2aff
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6748
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignments of the Ki67FHA/hNIFK(226-269)3P complex

Deposition date:

2005-07-25

Original release date:

2007-01-29

Authors:

Byeon, In-Ja; Gronenborn, Angela; Li, Hongyuan; Tsai, Ming-Daw

Citation:

Citation: Byeon, In-Ja; Li, Hongyuan; Song, Haiyan; Gronenborn, Angela; Tsai, Ming-Daw. "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67." Nat. Struct. Mol. Biol. 12, 987-993 (2005).

Assembly members:

Assembly members:
Ki67FHA, polymer, 120 residues, Formula weight is not available
hNIFK, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ki67FHA: MWPTRRLVTIKRSGVDGPHF PLSLSTCLFGRGIECDIRIQ LPVVSKQHCKIEIHEQEAIL HNFSSTNPTQVNGSVIDEPV RLKHGDVITIIDRSFRYENE SLQNGRKSTEFPRKIREQEP
hNIFK: KTVDSQGPTPVCTPTFLERR KSQVAELNDDDKDDEIVFKQ PISC

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	520
15N chemical shifts	168
1H chemical shifts	1055

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ki67FHA	1
2	hNIFK	2

Entities:

Entity 1, Ki67FHA 120 residues - Formula weight is not available

1	MET	TRP	PRO	THR	ARG	ARG	LEU	VAL	THR	ILE
2	LYS	ARG	SER	GLY	VAL	ASP	GLY	PRO	HIS	PHE
3	PRO	LEU	SER	LEU	SER	THR	CYS	LEU	PHE	GLY
4	ARG	GLY	ILE	GLU	CYS	ASP	ILE	ARG	ILE	GLN
5	LEU	PRO	VAL	VAL	SER	LYS	GLN	HIS	CYS	LYS
6	ILE	GLU	ILE	HIS	GLU	GLN	GLU	ALA	ILE	LEU
7	HIS	ASN	PHE	SER	SER	THR	ASN	PRO	THR	GLN
8	VAL	ASN	GLY	SER	VAL	ILE	ASP	GLU	PRO	VAL
9	ARG	LEU	LYS	HIS	GLY	ASP	VAL	ILE	THR	ILE
10	ILE	ASP	ARG	SER	PHE	ARG	TYR	GLU	ASN	GLU
11	SER	LEU	GLN	ASN	GLY	ARG	LYS	SER	THR	GLU
12	PHE	PRO	ARG	LYS	ILE	ARG	GLU	GLN	GLU	PRO

Entity 2, hNIFK 120 residues - Formula weight is not available

1

LYS

THR

VAL

ASP

SER

GLN

GLY

PRO

THR

PRO

2

VAL

CYS

THR

PRO

THR

PHE

LEU

GLU

ARG

3

LYS

SER

GLN

VAL

ALA

GLU

LEU

ASN

ASP

4

ASP

LYS

ASP

GLU

ILE

VAL

PHE

LYS

GLN

5

PRO

ILE

SER

CYS

Samples:

sample_1: Ki67FHA, [U-13C; U-15N], 1 mM; hNIFK 1 mM

sample_2: Ki67FHA 1 mM; hNIFK, [U-13C; U-15N], 1 mM

conditions_1: pH: 7.4; temperature: 290 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

DBJ	BAB41210 BAF84592 BAI47080
GB	AAH12457 AAH22990 AAH24238 AAY14830 ABM82604
REF	NP_115766 XP_003818352 XP_004031768 XP_009242042 XP_515769
SP	Q9BYG3
AlphaFold	Q9BYG3