BMRB Entry 6747
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6747
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Citation: Wu, Bin; Yee, Adelina; Lukin, Jonathan; Semesi, Anthony; Lemak, Alexander; Ramelot, Theresa; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of 50S ribosomal protein L40e from Sulfolobus solfataricus" The BMRB entry is the only known published source for the data..
Assembly members:
50S ribosomal protein L40e, polymer, 56 residues, 6444 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2303 Superkingdom: Archaea Kingdom: not available Genus/species: sulfolobus solfataricus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET15B
Entity Sequences (FASTA):
50S ribosomal protein L40e: MPLTDPAKLQIVQQRVFLKK
VCRKCGALNPIRATKCRRCH
STNLRLKKKELPTKKG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 383 |
| 13C chemical shifts | 235 |
| 15N chemical shifts | 47 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | sso5336 | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, sso5336 56 residues - 6444 Da.
| 1 | MET | PRO | LEU | THR | ASP | PRO | ALA | LYS | LEU | GLN | ||||
| 2 | ILE | VAL | GLN | GLN | ARG | VAL | PHE | LEU | LYS | LYS | ||||
| 3 | VAL | CYS | ARG | LYS | CYS | GLY | ALA | LEU | ASN | PRO | ||||
| 4 | ILE | ARG | ALA | THR | LYS | CYS | ARG | ARG | CYS | HIS | ||||
| 5 | SER | THR | ASN | LEU | ARG | LEU | LYS | LYS | LYS | GLU | ||||
| 6 | LEU | PRO | THR | LYS | LYS | GLY |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: 50S ribosomal protein L40e, [U-13C; U-15N], 1 mM; NaCl 450 mM; Phosphate 25 mM
cond_set1: pH: 6.5; temperature: 298 K; ionic strength: 0.45 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | sample_1 | not available | cond_set1 |
| HNCO | sample_1 | not available | cond_set1 |
| HNcaCO | sample_1 | not available | cond_set1 |
| CBCAcoNH | sample_1 | not available | cond_set1 |
| HNCB | sample_1 | not available | cond_set1 |
| HNCA | sample_1 | not available | cond_set1 |
| HNcoCA | sample_1 | not available | cond_set1 |
| HBHAcoNH | sample_1 | not available | cond_set1 |
| CCTOCSY_NH | sample_1 | not available | cond_set1 |
| HCCTOCSY_NH | sample_1 | not available | cond_set1 |
| HNHA | sample_1 | not available | cond_set1 |
| 13C HSQC | sample_1 | not available | cond_set1 |
| HCCHCOSY | sample_1 | not available | cond_set1 |
| 15N NOESY | sample_1 | not available | cond_set1 |
| 13C NOESY | sample_1 | not available | cond_set1 |
Software:
SPARKY v3.95 - spectral analysis, assignemt
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks