BMRB Entry 6747

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6747

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Assignment of 50S ribosomal protein L40e
Deposition date:
2005-07-25
Original release date:
2006-02-23
Authors:
Wu, Bin; Yee, Adelina; Lukin, Jonathan; Semesi, Anthomy; Lemak, Alexander; Ramelot, Theresa; Kennedy, Michael; Arrowsmith, Cheryl
Citation:

Citation: Wu, Bin; Yee, Adelina; Lukin, Jonathan; Semesi, Anthony; Lemak, Alexander; Ramelot, Theresa; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of 50S ribosomal protein L40e from Sulfolobus solfataricus"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
50S ribosomal protein L40e, polymer, 56 residues, 6444 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2303   Superkingdom: Archaea   Kingdom: not available   Genus/species: sulfolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET15B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
50S ribosomal protein L40e: MPLTDPAKLQIVQQRVFLKK VCRKCGALNPIRATKCRRCH STNLRLKKKELPTKKG

Data sets:
Data typeCount
1H chemical shifts383
13C chemical shifts235
15N chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sso53361
2ZINC (II) ION2

Entities:

Entity 1, sso5336 56 residues - 6444 Da.

1   METPROLEUTHRASPPROALALYSLEUGLN
2   ILEVALGLNGLNARGVALPHELEULYSLYS
3   VALCYSARGLYSCYSGLYALALEUASNPRO
4   ILEARGALATHRLYSCYSARGARGCYSHIS
5   SERTHRASNLEUARGLEULYSLYSLYSGLU
6   LEUPROTHRLYSLYSGLY

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: 50S ribosomal protein L40e, [U-13C; U-15N], 1 mM; NaCl 450 mM; Phosphate 25 mM

cond_set1: pH: 6.5; temperature: 298 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
15N HSQCsample_1not availablecond_set1
HNCOsample_1not availablecond_set1
HNcaCOsample_1not availablecond_set1
CBCAcoNHsample_1not availablecond_set1
HNCBsample_1not availablecond_set1
HNCAsample_1not availablecond_set1
HNcoCAsample_1not availablecond_set1
HBHAcoNHsample_1not availablecond_set1
CCTOCSY_NHsample_1not availablecond_set1
HCCTOCSY_NHsample_1not availablecond_set1
HNHAsample_1not availablecond_set1
13C HSQCsample_1not availablecond_set1
HCCHCOSYsample_1not availablecond_set1
15N NOESYsample_1not availablecond_set1
13C NOESYsample_1not availablecond_set1

Software:

SPARKY v3.95 - spectral analysis, assignemt

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

REF NP_341727
SWISS-PROT Q980V5
PDB
GenBank AAK40517

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks